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EC number: 295-415-9 | CAS number: 92045-34-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May to October 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: the study was performed according to OECD guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Instead of maximal 1000 mg/L the concentration range in the screening test was set at up to 100 mg/L. This represents a deviation of the study plan which is considered to have no influence on the integrity of the study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- The values >21°C are slightly out of the temperature range set at 20 ± 1°C. This represents a deviation of the study plan which is not considered to have an influence on the integrity of the study.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Absorbent A4
- IUPAC Name:
- Absorbent A4
- Details on test material:
- - Name of test material (as cited in study report): Absorbent A4
- Substance type: UVCB
- Physical state: Transparent liquid without mechanical impurities, colour from light to dark
- Stability under test conditions: months
- Storage condition of test material: room temperature, in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 12.8 mg / 2000 mL, 25.0 mg / 2000 mL, 49.9 mg / 2000 mL, 100.8 mg / 2000 mL, and 200.3 mg / 2000 mL
- Sampling method: data not available
- Sample storage conditions before analysis: data not available
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test item was tested for toxicity to¬wards daphnia as aqueous extracts (“WAF”) from up to 100 mg test item/L (final concentration). For this purpose, different amounts of the test item were dispersed in dilution water. The elution of the water soluble part (WAF) of the test item took place on a shaking machine at 130 rpm speed over 24 h at room temperature, respectively. During that time an equilibration between the test item and the water was considered to be achieved. Thereafter, the suspensions were filtered through glass fibre filters in order to eliminate non-dissolved parts of the test item. The eluates were used for the test without further dilution steps.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna STRAUS (clone 5)
- Source: Of a laboratory breed (derived from Dr. M. Bergtold, BASF, D-67117 Limburgerhof; the strain originally derived from the Institut National de Recherche Chimique Appliquee, France in 1978 and cultivated since then at BASF using medium M4 acc. Elendt) was used for the test.
- Age at study initiation: not older than 24h
- Method of breeding: the cultivation of the daphnias is performed in a way that the animals are transferred in new test medium at an interval of 1 to 3 days followed by feeding with Desmodesmus subspicatus- and/or Chlorella-cells.
- Feeding during test: during the test the daphnia were not fed
ACCLIMATION: data not available
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- data not available
- Test temperature:
- see table 1
- pH:
- see table 1
- Dissolved oxygen:
- see table 1
- Salinity:
- not applicable (freshwater)
- Nominal and measured concentrations:
- see table 2 and 3
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL carboys made of glass
- Type (delete if not applicable): data not available
- Aeration: data not available
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: data not available
TEST MEDIUM / WATER PARAMETERS: test medium according Elendt M4 according to the guideline (prepared in ultra pure water)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h of light and 8h of darkness
- Light intensity: data not available
EFFECT PARAMETERS MEASURED: after 24h and 48h the number of immobilised daphnia at each concentration of the test item is recorded
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: 1 mg/L, 10 mg/mL and 100 mg/L
- Results used to determine the conditions for the definitive study: no immobilisation observed at the concentration of 1 mg/L - Reference substance (positive control):
- no
- Remarks:
- A reference item was not used within the biological part of this study. Within the analytical part of the study, the test item served as the reference.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EL10
- Effect conc.:
- 1.56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 1.81 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.98 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.84 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- In order to verify the sensitivity of the test system towards toxic items the effect of potassium dichromate towards different animals of this breed was tested prior to beginning of the test. The value of the EC50 (24h) in this period of time was 1.06 mg/L potassium dichromate. This value is in accordance with the recommendations given in OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test).
- Reported statistics and error estimates:
- not applicable
Any other information on results incl. tables
Main Test
Nominal Concentration of the testitem(mg/L) (Eluates/“WAF“) |
Ratio immobilised Daphnia [%] |
pH-Value
|
O2-Content [mg O2/L] |
Temperature*)[°C] |
|||||
24h |
48h |
t0 |
48h |
t0 |
48h |
t0 |
48h |
||
Control (0) |
0 [0/20]1) |
0 [0/20]1) |
8.74 |
8.87 |
8.6 |
8.4 |
21.1 |
20.7 |
|
6.25 |
|
0 [0/20]1) |
0 [0/20]1) |
8.63 |
8.70 |
7.2 |
7.5 |
21.0 |
20.8 |
12.50 |
|
20 [4/20]1) |
55 [11/20]1) |
8.71 |
8.78 |
6.7 |
7.4 |
21.0 |
20.8 |
25.00 |
|
100 [20/20]1) |
100 [20/20]1) |
8.63 |
8.75 |
6.1 |
6.5 |
21.1 |
20.5 |
50.00 |
|
100 [20/20]1) |
100 [20/20]1) |
8.78 |
8.85 |
5.7 |
6.0 |
21.1 |
20.6 |
100.00 |
|
100 [20/20]1) |
100 [20/20]1) |
8.68 |
8.79 |
6.1 |
6.4 |
21.1 |
20.7 |
1)Numbers in brackets give the absolute numbers of immobilised animals at each concentration.
Effective Concentrations on Basis of the Nominal Concentrations:
t24h |
t48h |
|
NOEC |
12.5 mg/L |
6.25 mg/L |
LOEC |
25.0 mg/L |
12.5 mg/L |
EC10 |
11.8 mg/L |
10.3 mg/L |
EC20 |
12.5 mg/L |
11.0 mg/L |
EC50 |
13.9 mg/L |
12.3 mg/L |
Effective Concentrations (Loadings) on Basis of the Measured Concentrations:
t24h |
t48h |
|
NOEC |
1.84 mg/L |
0.98 mg/L |
LOEC |
3.74 mg/L |
1.84 mg/L |
EC10 |
1.75 mg/L |
1.56 mg/L |
EC20 |
1.84 mg/L |
1.65 mg/L |
EC50 |
2.02 mg/L |
1.81 mg/L |
*)Temperatures were partially outside the range of 20 ± 1°C indicated in the study plan. This represents a deviation of the study plan which is not considerd to have an influence on the integrity of this study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- In the control without any test item no dead animals were observed. In the test solutions the oxygen concen¬tration did not drop below a value of 60% of the saturation value (ca. 5 mg O2/L).
- Conclusions:
- The reported effect concentrations refer to mean measured concentrations (geometric mean) of the measured concentrations. The following EC-values (effective concentrations) were determined acordingly:
t24h t48h
NOEC 1.84 mg/L 0.98 mg/L
LOEC 3.74 mg/L 1.84 mg/L
EC10 1.75 mg/L 1.56 mg/L
EC20 1.84 mg/L 1.65 mg/L
EC50 2.02 mg/L 1.81 mg/L - Executive summary:
”Absorbent A4“was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to investigate the influence of the test item towards the daphnias, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnias was observed at concentrations representing the water soluble part (hereafter abbreviated “WAF”, water accommodated fraction) of the test item at concentrations up to 100 mg/L (screening test). As a conclusion of the analytical part of this study, recoveries of the concentrations determined after 48h were not all at ≥ 80% of those determined at time t0, and furthermore,the measured concentrations of the treatments deviated more than 20 % from the nominal values. Based on this, the reported effect concentrations refer tomean measuredconcentrations (geometric mean) of the measured concentrations.The following EC-values (effective concentrations) were determined acordingly:
t24h
t48h
NOEC
1.84 mg/L
0.98 mg/L
LOEC
3.74 mg/L
1.84 mg/L
EC10
1.75 mg/L
1.56 mg/L
EC20
1.84 mg/L
1.65 mg/L
EC50
2.02 mg/L
1.81 mg/L
The test is considered valid as all conditions for validity were met.
· In the control without any test item no dead animals were observed.
· In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (»5 mg O2/L).
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