Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1998

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-chloro-3,5-dinitro-α,α,α-trifluorotoluene
EC Number:
206-889-3
EC Name:
4-chloro-3,5-dinitro-α,α,α-trifluorotoluene
Cas Number:
393-75-9
Molecular formula:
C7H2ClF3N2O4
IUPAC Name:
4-chloro-3,5-dinitro-α,α,α-trifluorotoluene

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg/l
Test temperature:
20-21°C
pH:
8.0-8.1
Nominal and measured concentrations:
Due to the low water solubility a supersaturated stock suspension of the test item with a nominal concentration of 100 mg/l was continuously stirred at room temperature in the dark over 96 hours and filtered. The dilutions 1:100, 1:222, 1:454, 1:1000 and 1:2222 of the filtrate were used as test media.
The mean analytically determined test item concentration in the analyzed test media samples (dilutions 1:454, 1:222 and 1:100) amounted to 0.18, 0.38 and 0.88 mg/l at the start of the test. The values found at the end of the test ranged from 98-106% of the initially measured values.
The lowest test concentrations (dilutions 1:1000 and 1:2222) were not analysed since below the 48-hr NOEC.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.6 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.5-0.7 mg/l
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.38 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
1.9 mg/L
Nominal / measured:
estimated
Details on results:
All biological test results are related to the total mean measured test item concentrations of 0.19 mg/l (1:454), 0.38 mg/l (1:222) and 0.87 mg/l (1:100), calculated as the average over all measurements per test concentration during the test period.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes