1-Propanol, 3-(dimethylamino)-, hydrochloride (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-(dimethylamino)propan-1-ol hydrochloride

EC Number:

601-495-1

Cas Number:

117829-74-0

Molecular formula:

C5 H13 N O. Cl H

IUPAC Name:

3-(dimethylamino)propan-1-ol hydrochloride

Details on test material:

- Name of test material (as cited in study report): 3-Dimethylamino-Propanol-Hydrochlorid-1
- Batch no.: KT 215
- Substance no.: 00/0616-1
- Product no.: 63181
- Aggregated state: liquid
- Water-solubility (mg/L): at least 500
- Colour: colourless, clear
- Homogeneity: yes
- Stability: stability of test substance is guaranteed by analytical report no. 01L00144 from April 30th, 2001
- Further remarks: test substance was characterized by H-NMR spectroscopy

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Activated sludge from laboratory wastewater plants treating municipal sewage
- Concentration of dry substance: 30 mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

20 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

70 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST INFORMATION
- Test temperature: 22 +/- 2 °C

TEST CONDITIONS
pH-values before adding inoculum and after correction: 7.4

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

90 - 100

Sampling time:

28 d

Details on results:

KINETIC OF TEST SUBSTANCE MEAN (%):
= 12 after 1 day
= -1 after 3 days
= 7 after 5 days
= 9 after 7 days
= 13 after 10 days
= 20 after 14 days
= 40 after 17 days
= 88 after 21 days
= 98 after 24 days
= 99 after 27 days
= 100 after 28 days

BOD5 / COD results

Results with reference substance:

- DOC decrease (%) after 14 days: 91

Any other information on results incl. tables

TEST INFORMATION

- Duration of adaption phase (d): 8

- Duration of degradation phase (d): 16

- Elimination of test substance by adsorption (% DOC): <10 after 5 d

- Physico-chemical (abiotic) elimination of test substance (% DOC): <10 at end of test

Applicant's summary and conclusion

Interpretation of results:

readily biodegradable, but failing 10-day window