1-Propanol, 3-(dimethylamino)-, hydrochloride (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Test guideline 4

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

EEC Directive 92/32/EEC Annex V, part C

Test guideline 5

Qualifier:

according to guideline

Guideline:

other: International Standard ISO/DIS 10706 (Water quality - Determination of long term toxicity of substances to Daphnia magna STRAUS)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-(dimethylamino)propan-1-ol hydrochloride

EC Number:

601-495-1

Cas Number:

117829-74-0

Molecular formula:

C5 H13 N O. Cl H

IUPAC Name:

3-(dimethylamino)propan-1-ol hydrochloride

Details on test material:

- name of test material (as cited in study report): 3-Dimethylamino-Propanol-Hydrochlorid-1
- batch number: KT 215
- product number : 63181
- aggregate state: liquid
- water-solubility (mg/L): 100
- colour: colourless-clear
- homogeneity: yes
- stability: stability of test substance over test period guaranteed by sponsor
- storage conditions: ambient

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

- test substance stirred in M4 medium for ca. 10 minutes
- nominal concentration of stock solution: 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnia magna STRAUS 1820 clone was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. Since then, clone was
cultured and bred continuously in BASF's Laboratory Experimental Toxicology and Ecology in Ludwigshafen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.4 - 20.6 °C

pH:

- pH range at start of test : 7.7 - 8.0
- pH range at end of test: 8

Dissolved oxygen:

- oxygen range at start of test (mg/L): 8.6 - 8.9
- oxygen range at end of test (mg/L): 8.4 - 8.6

Nominal and measured concentrations:

- concentrations (mg/L): 12.5, 25, 50, 100 (nominal)
- analytical recovery rate of > 80 %

Details on test conditions:

TEST SYSTEM
- Test vessel: test tubes (glass) with flat bottom (nominal volume 20 ml)
- Test volume: 10
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of animals per concentration: 20
- Loading (animals/ml): 0.5
- No. of replicates for the measurement of pH and O2: 1
- No. of parallels without animals: 1 per test concentration and control for measurement of pH and O2 content at beginning of test. 2 per
concentration and control for concentration control analysis

TEST MEDIUM PARAMETERS
- Alkalinity up to pH 4.3: 0.8 - 1 mmol/L
- Molar ratio Ca/Mg: ca. 4:1
- Conductivity (µS/cm) 550 - 650
- Culture medium different from test medium: no
- Total hardness (mmol/L): 2.20 - 3.20
- after preparation, M4 medium is aerated for ca. 24h until oxygen saturation is reached

OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm = 16:8h
- Light intensity (µE/(m2*s)): ca. 2 - 7 at wave length of 400 - 700 nm
- Illumination: artificial light, type warm white

TEST ORGANISM
- Age of animals at start of test: 2 - 24h (starting with 3rd breed of parent animals)
- Age of stock animals: 2 - 4 weeks
- Feeding: none

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Test parameter: swimming ability of test animals
- check of the study and recording: visually after 0, 24 and 48h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

duration 24h EC0 >=100 nominal test mat. mobility
duration 24h EC50 > 100 nominal test mat. mobility
duration 24h EC100 > 100 nominal test mat. mobility

Any other information on results incl. tables

Analytically determined concentrations of the test substance in the test solutions. Values are given in percent of nominal concentrations:

Time     Daphnia    Values in % of nominal concentration
[h]                            Min               Max
-----------------------------------------------------------
0           no               100              100
48         no               97.6             100

With an analytical recovery rate of > 80 %, no transformation of the nominal EC-values into the values of the concentration control analysis occurs. ANALYTICAL RESULTS0h    expected concentration in sample: 0 mg/L         value found: <5 mg/L0h    expected concentration in sample: 12.5 mg/L   value found: 12.5 mg/L0h    expected concentration in sample: 100 mg/L    value found: 100 mg/L48h  expected concentration in sample: 0 mg/L        value found: <5 mg/L48h  expected concentration in sample: 12.5 mg/L   value found: 12.2 mg/L48h  expected concentration in sample: 100 mg/L    value found: 100 mg/L