2-Naphthalenecarboxylic acid, 6-[[[4-[(1-oxo-2-propen-1-yl)oxy]butoxy]carbonyl]oxy]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP-compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Test guideline 4

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Test guideline 5

Qualifier:

according to guideline

Guideline:

other: ISO/DIS 10706 (Water quality - Determination of long term toxicity of substances to Daphnia magna STRAUS)

GLP compliance:

yes (incl. QA statement)

Remarks:

Experimental Toxicology and Ecology, BASF Aktiengesellschaft, 67056 Ludwigshafen/Rhein, Germany

Test material

Test material information

Constituent 1

Reference substance name:

297132-04-8

EC Number:

608-403-9

Cas Number:

297132-04-8

IUPAC Name:

297132-04-8

Details on test material:

- Name of test substance: 4-(4-Acryloyloxy-butoxycarbonyloxy)-benzoic acid
- Aggregate state/colour: solid / white
- Homogeneity: homogeneous
- Storage conditions: Store at room temperature under light exclusion. Keep dry

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 12.5, 25, 50, 100 mg/L
- Sampling method: At the begin (after 0 h) of the exposition samples from vessels without daphnids were analysed. At the end of the exposition (after 48 h) samples from vessels with daphnids were analysed.
- Sample storage conditions before analysis: room temperature

Test solutions

Vehicle:

no

Details on test solutions:

All test concentrations were weight in separately in M4 medium. The concentrations were treated in an ultrasonic bath for approx. 5 minutes and then stirred for about 20 hours at 20± 2°C. Undissolved test substance was removed by centrifugation (approximately 20 min at about 17700 G).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in-house.
- Age of the animals at the begin of exposition: 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals: 2 - 4 weeks

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.39 mmol/L

Test temperature:

19.7 - 20.2°C

pH:

7.5 - 8.1

Dissolved oxygen:

8.2 - 9.3 mg/L

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 12.5, 25, 50, 100 mg/L
- Measured concentrations at t=0h: 12.78, 23.1, 49.8, 93.5 mg/L
- Measured concentrations at t=24h: 11.92, 22.1, 47.8, 90.8 mg/L

Details on test conditions:

- Test vessel: Test tubes (glass) with flat bottom (nominal volume 20 mL)
- Test volume: 10mL
- Loading (animals/mL): 0.5
- Number of animals/vessel: 5
- Total number of animals/conc.: 20
- Number of replicates/conc.: 4
- Number of replicates/control: 4
- Number of replicates for the measurement of pH and O2 : 1
- Number of parallels without animals: 1 per test concentration and control for measurement of the pH and oxygen content at the beginning of the exposition. This parallel was thrown away after measurement. 4 per test concentration and control for concentration control analysis.
- Illumination: Artificial light, type warm white (OSRAM L58 W31); day:night rhythm = 16 : 8 h
- Light intensity: About 1 - 8 µE/(m2.s) at a wave length of 400 - 750 nm
- Test parameter: Swimming ability of the test animals
- Check of the study and recording (swimming ability): Visually after 0, 24 and 48 h
- Measurement of temperature: Continuously during the whole test period in a separate vessel close to the test vessels.
- Measurement of pH and oxygen: After 0h in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility