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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16 april 2013 to 26 april 2013
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Reaction mass of disodium 2-{[2-(carbamoylamino)-4-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)phenyl]diazenyl}-5-(vinylsulfonyl)benzenesulfonate and trisodium 2-{[2-(carbamoylamino)-4-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)phenyl]diazenyl}-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate
IUPAC Name:
Reaction mass of disodium 2-{[2-(carbamoylamino)-4-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)phenyl]diazenyl}-5-(vinylsulfonyl)benzenesulfonate and trisodium 2-{[2-(carbamoylamino)-4-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)phenyl]diazenyl}-5-{[2-(sulfonatooxy)ethyl]sulfonyl}benzenesulfonate
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
in Elendt-M4-medium, pre-aereated for 2 hours

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
20.1 - 21.6° C
pH:
pH 7.0 - 7.2
Dissolved oxygen:
8.2 - 7.9
Nominal and measured concentrations:
nominal: 100 mg/L; measured concentrations between 102.7 and 105.0 mg/L.
max deviation: 4.9 %
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Results with reference substance (positive control):
The last quality check was performed in November 2012:
The EC50 (24h) was determined as 1.92 mg/L (95% - confidence interval: 2.55 – 3.97 mg/L).
The EC50 (48h) was determined as 1.50 mg/L (95% - confidence interval: 1.42 – 1.73 mg/L).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
With the test solution of 100 mg/L of the substance the following result was achieved:
EC50 (24h, nominal concentration): > 100 mg/L
EC50 (48h, nominal concentration): > 100 mg/L.