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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: no guideline study, test concentrations not specified, read across

Data source

Reference
Reference Type:
publication
Title:
METABOLlSM OF TOXIC DOSES OF o-CHLOROBENZYLIDENE MALONONITRILE (CS) IN THE RABBlTS
Author:
Paradowski M.
Year:
1979
Bibliographic source:
Pol.J.Pharmacol.Pharm., 31 (1979) 563-572

Materials and methods

Test guideline
Qualifier:
no guideline available
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
o-chlorobenzylidene malononitrile (CS)
IUPAC Name:
o-chlorobenzylidene malononitrile (CS)
Details on test material:
- Name of test material (as cited in study report): o-chlorobenzaldehyde
- Analytical purity: min. 98% (distilled freshly prior to use)

Test animals

Species:
rabbit
Strain:
other: Mongrel
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.4 - 3.2 kg
- Fasting period before study: 15h
- Diet (e.g. ad libitum): standard food for rabbits type LSK and fresh vegetables

Administration / exposure

Route of administration:
intravenous
Vehicle:
DMSO
Doses:
4 dose levels
No. of animals per sex per dose:
4 animals
Control animals:
no
Details on study design:
- Duration of observation period following administration: 24 h

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 8.5 mg/kg bw
Based on:
test mat.

Applicant's summary and conclusion

Conclusions:
Regarding the results of this study, the LD50 for the test substance o-chlorobenzaldehyde after intravenous administration in rabbits is considered to be 8.5 mg/kg bw per day. The study has been judged not to be reliable due to insufficient documentation.