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Administrative data

Key value for chemical safety assessment

Additional information

In a Salmonella/microsome assay for mutagenicity (without preincubation) performed with Salmonella typhimurium TA 98, 100, 1535 and 1537 no mutagenic effect appeared after exposure to 2-chlorobenzaldehyde with and without metabolic activation (S9) Zeiger et al., 1992).

Furthermore, a second Salmonella/microsome assay for mutagenicity (without preincubation) performed with Salmonella typhimurium TA 100 according to Ames et al . (1975) also revealed no mutagenic effect after exposure to the test substance 2 -chlorobenzaldehyde (Rietveld et al., 1983).

2-chlorobenzaldehyde was examined for potential mutagenicity in the Salmonella/ mammalian-microsome assay with and without adding a metabolic activating system (S9). In both cases the test substance showed no mutagenic effect (Nestmann et al., 1980).

Ziegler-Skylakakis et al. (1989) investigated the genotoxicity of 2-chlorobenzaldehyde to V79 Chinese hamster cells using the induction of micronuclei as a biological endpoints. Furthermore the cytotoxicity of o-Chlorobenzaldehyde was measured by determining the cloning efficiencies of treated and untreated cells immediately after exposure to the test substance. 2-chlorobenzaldehyde did not cause a significant increase in the frequency of micronuclei up to the highest concentration tested and the cell survival of the V79 cells was only slightly reduced (15%).

The clastogenicity of 2-chlorobenzaldehyde to V79 Chinese hamster cells was investigated after a 20 hour-exposure. O-chlorobenzaldehyde was inactive in these experiments and did not show a clastogenic effect (Bauchinger and Schmid, 1992).

When V79 cells were exposed to 2-chlorobenzaldehyde for 20 h and incubated without the test substance for another 20 h, o-chlorobenzaldehyde was found to be highly aneuploidogen; as compared to the control, a 6-fold higher incidence of aneuploid cells was observed, but the effect was not dose dependent (Schmid and Bauchinger, 1991).


Short description of key information:
Genetic toxicity:
-in vitro:
-Ames test: Zeiger et al. (1992), similar or equivalent to OECD guideline 471 - not genotoxic, not mutagenic.
Rietveld et al. (1983), similar or equivalent to OECD guideline 471 - not genotoxic, not mutagenic.
Nestmann et al. (1980), bacterial reverse mutation assay as described in the literature (Ames et al., 1975) - not genotoxic, not mutagenic.
-In vitro mammalian cell micronucleus test: Ziegler-Skylakakis et al. (1989), similar or equivalent to OECD guideline 487 - not genotoxic, not mutagenic.
- Clastogenic capacity: Bauchinger and Schmid (1992) - not clastogenic
- Analysis of the aneuploidy inducing capacity: Schmid and Bauchinger (1991) - highly aneuploidogen.
- in vivo: no data

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

The Ames Test, as well as the in vitro mammalian cell micronucleus test and a chromosome aberration test revealed neither genotoxic nor mutagenic effects of 2-chlorobenzaldehyde in bacteria or mammalian cells in vitro. Only one publication showed aneuploidy and polyploidy in V79 Chinese hamster cells. As the majority of the published studies showed no signs of mutagenicity, the test substance 2 -chlorobenzaldehyde is considered no to be mutagenic.

Data of in vivo genotoxicity and mutagenicitiy tests were not available to date.