Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-07-08 to 1991-07-12
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study, no deficiencies

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Lot No. E 011 204 60
ca. 80% solid test item
ca. 10% Olefin
ca. 10% Water
brown liquid
pH 8.2 at 10 g/L

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Stock solution 1000 mg/L (opaque but homogenous)

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Source: Zoo-Stumpe, Hildesheim
Feed: Tetramin
Length: average 3.1 cm
Weight: average 0.36 g

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
not given
Test temperature:
23 +/- 2 deg C
pH:
7.82-8.03
Dissolved oxygen:
Saturation degree: 97-99%
Nominal and measured concentrations:
nominal: Control-10-18-32-58-100 mg/L
Details on test conditions:
Glas aquarium: 15L
10 fish per concentration
Light: diffus 12-16 h day light
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CI 49-68 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance was tested in an OECD 203 acute Fish test and from the results a LC50(96) of 58 mg/L was derived.