N6-(1-oxooctyl)-L-lysine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Fraunhofer IME

Test material

Test material information

Constituent 1

Reference substance name:

N6-(1-oxooctyl)-L-lysine

EC Number:

245-854-7

EC Name:

N6-(1-oxooctyl)-L-lysine

Cas Number:

23735-96-8

Molecular formula:

CH3-(CH2)6-CO-NH-(CH2)4-CH(-NH2; -COOH)

IUPAC Name:

(2S)-2-amino-6-octanamidohexanoic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Acylglycinate GCK-11-T
- Physical state: White to pale yellow powder, a slightly characteristic odor
- Lot/batch No.: 10825
- Expiration date of the lot/batch: August 25, 2014
- Storage condition of test material: Dark and cool place, protected from light
- ThoD: 2.060 mg O2/mg

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, Germany. Mainly fed with municipal wastewater
- Method of cultivation: samples were not washed with mineral medium after the arrival at the laboratory but kept aerobic until use
- Initial cell/biomass concentration: 29.6 mg dry mass/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

100 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

206 mg/L

Based on:

ThOD/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 22 °C ± 1°C
- pH: 7.2 - 7.8
- pH adjusted: yes

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes (sterilized with HgCl2)
- Toxicity control: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

100 mg/L

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

85.9

Sampling time:

14 d

Remarks on result:

other: mean of 2 determinations

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

93.7

Sampling time:

28 d

Remarks on result:

other: mean of 2 determinations

Any other information on results incl. tables

The biodegradation within the 10-day-window was 82.5 %. The 10-day-window starts at day 1.

No significant abiotic degradation of Acylglycinate GCK-11-T was noticeable after 28 days of incubation.

The biodegradation of the item mixture in the toxicity control was found to be 87.9 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item can be identified as non-toxic in a ready biodegradability test.

The reference item sodium benzoate was degraded to 88.6 % within the first 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable