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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
Nickel, compound with Titanium (1:1)
EC Number:
Cas Number:
Molecular formula:
Nickel, compound with Titanium (1:1)
Details on test material:
100% Equiatomic NiTi intermetallic compound
Batch 1048

Sampling and analysis

Analytical monitoring:

Test solutions

Details on test solutions:
The test item has been prepared as a stck solution (10 g/l) and then this solution and 4 different concentrations in water (max solubility) have been tested. Because the test item is poorly soluble in water to facilitate its solubility in water the stock solution has been stirred for 70 hours and then before the beginning of the test this solution has been ultrasonicated for 30 minutes.
The total volume of the culture medium and the concentration of the algae inoculum added are the same in all vessels.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
- Family: chlorophyceae
- order: chlorococcalles
- culturing: the algal suspension has been kept in culture medium in sterile 250 ml Erlenmeyer flasks at 23 +/- 2 °C, with lighting greater than 4300 lux and steady with electrical stirrer

Study design

Test type:
not specified
Water media type:
Limit test:
Total exposure duration:
72 h
Post exposure observation period:
Every 24 hours from the beginning of the test until the end of the test (72 hours), cellular concentration has been determined, by means of Burker chamber, of treated and of control organisms.

Test conditions

Test temperature:
23 +/- 2 °C
from 7.70 to 9
Nominal and measured concentrations:
Administered solutions (g/l): 10.00, 3.130, 0.980, 0.310, 0.095
Details on test conditions:
Because the test will done on the extract of the test item, constituted by metals with a low volatilisation from water to air, at the beginning and at the end of the test have been checked the concentrations of two metals (nichel and titanium) in the extract by ICP-OES.

Results and discussion

Effect concentrations
72 h
Dose descriptor:
Effect conc.:
> 10 g/L
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
A normal and healthy appearance of the algae at the end of the test was observed at microscopic.

Any other information on results incl. tables

             Algal density (cells/ml) in the treated group 10 g/l
  Time (days)  1  2  3
 0  10000   10000   10000
 1  32000  33000  32000
 2  260000  240000  210000
 3  630000  650000  620000

Applicant's summary and conclusion

Validity criteria fulfilled:
The obtained results after 72 hours showed that EC50 of the test item is greater than 10 g/l.