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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Qualifier:
according to guideline
Guideline:
EU Method B.3 (Acute Toxicity (Dermal))
GLP compliance:
yes
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
o-fluoro-α,α,α-trichlorotoluene
EC Number:
207-692-5
EC Name:
o-fluoro-α,α,α-trichlorotoluene
Cas Number:
488-98-2
Molecular formula:
C7H4Cl3F
IUPAC Name:
1-fluoro-2-(trichloromethyl)benzene

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Type of coverage:
semiocclusive
Vehicle:
unchanged (no vehicle)
Duration of exposure:
14 days
Doses:
The test article was applied at a dose of 2000 mg/kg b.w. evenly on the intact skin with a syringe and covered wita asemi-occlusive dressing.
Application volume/kg b.w.: 2.0 mL
No. of animals per sex per dose:
5 males
5 females
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No deaths occurred during the study.
Clinical signs:
The following local alteration of the skin at the application site was noted:
2000 mg/kg
Scales on the back (5/5)*
General erythema on the back (4/5)
Crusts on the back (1/0)

In addition, emaciation was observed in 2 male and 3 female rats.
* (/) = number of male/female animals with local skin alterations.
Body weight:
Four male and five female animals lost weight (between 0.9% and 8.6%) during the first week of observation. They recovered during the second observation week.
Other findings:
Macroscopic findings:
No macroscopic organ findings were noted at necropsy.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LD50 of test substance after single dermal administration to rats of both sexes, observed over a period of 14 days, could not be estimated as no death occurred.
LD50 is > 2000 mg/kg b.w.