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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 26 - December 22, 1998
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4-dichloro-α,α,α-trifluorotoluene
EC Number:
206-278-1
EC Name:
2,4-dichloro-α,α,α-trifluorotoluene
Cas Number:
320-60-5
Molecular formula:
C7H3Cl2F3
IUPAC Name:
2,4-dichloro-1-(trifluoromethyl)benzene
Details on test material:
- Name of test material (as cited in study report): 2,4 Dichloro Benzotrifluoride
- Substance type: insoluble in water
- Physical state: liquid
- Purity test date: 99.8%
- Lot/batch No.: 950020
- Expiration date of the lot/batch: May 1999
- Storage condition of test material: room temperature, away from direct sunlight

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: University of Sheffield UK
- Age at study initiation (mean and range, SD): 6-24 hours old, not first brood progeny
- Amount: 20 daphnids, divided into two groups

The daphnids were not fed and the test media were not areated during the test period.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
2.5 mmol/l (reconstitued water)
Test temperature:
water temperature: 20-21 °C
pH:
8.0 - 8.1
Dissolved oxygen:
8.3 mg/l

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.18 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.18 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Details on results:
No toxic effect up to the highest concentration which could be dissolved in the test water.
The mean analytically determined test concentration in the samples from the freshly prepared test medium amounted to 0.17 and 0.19 mg/l at the start and at the end of the test. All biological results are related to the total mean measured test item concentration of 0.18 mg/l (calculated as the average over all measurements in the undiluted filtrate during the test period).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item had no toxic effect on the daphnids up to highest test item concentration, which could be dissolved or very finely dispersed in the test water.

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