2,4-dichloro-α,α,α-trifluorotoluene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

study only performed with TA98 and TA100

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichloro-α,α,α-trifluorotoluene

EC Number:

206-278-1

EC Name:

2,4-dichloro-α,α,α-trifluorotoluene

Cas Number:

320-60-5

Molecular formula:

C7H3Cl2F3

IUPAC Name:

2,4-dichloro-1-(trifluoromethyl)benzene

Details on test material:

- Name of test material (as cited in study report): 2.4-Dichloro BTF
- Substance type: organic
- Physical state: liquid
- Analytical purity: 99.8%
- Lot/batch No.: 930150

Method

Target gene:

TA 98: his D 3052; rfa-; uvrB-; R-factor:frameshift-mutations
TA 100: his G 46; rfa-; uvrB-; R-factor:basepair substitutions

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 98

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9 Mix

Test concentrations with justification for top dose:

Exp. I: 33.3, 100.0, 333.3, 1000.0, 2500.0 and 5000.0 ug/plate
Exp. II: 33.3, 66.6, 100.0, 333.3, 1000.0 and 2500.0 ug/plate

Controls

Untreated negative controls:

yes

Remarks:

concurrent untreated and solvent controls

Positive controls:

yes

Positive control substance:

other: without S9 mix sodium azide for TA100 and 4-nitro-o-phenylene-diamine for TA98. With S9 mix 2-aminoanthracene, 2-AA for both strains

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at highest concentrations

Test results 2

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at highest concentrations

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

No substantial increases in revertant colony numbers of any of the two tester strains were observed following treatment with the substance at any dose level, with or without metabolic activation (S9 mix). There was also no tendency of higher mutation rates with increasing concentrations in the range below the generally acknowledged border of significance.