Chloroacetic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

6 Jun 1975 - 24 Mar 1976

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Basic information is available

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Circular on Test Methods of New Chemical Substances (Japan), Biodegradation test

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Chloroacetic acid

EC Number:

201-178-4

EC Name:

Chloroacetic acid

Cas Number:

79-11-8

Molecular formula:

C2H3ClO2

IUPAC Name:

chloroacetic acid

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Chloroacetic acid
- Molecular formula (if other than submission substance): C2H2O2Cl
- Analytical purity: No data

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

m

Duration of test (contact time):

3 wk

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation 3

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- pH adjusted: yes

TEST SYSTEM
- Culturing apparatus: closed aotumatic oxgen consmuption aparatus
- Number of culture flasks/concentration: 2 abiotic control (water + test substance), 2 test suspenion (sludge + test substance), 1 inoculum blank (sludge) adn 2 medium blank (water)
- Measuring equipment: O2 control, TOC and GC

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Reference substance

Reference substance:

not specified

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

65

Sampling time:

3 wk

% Degradation 2

Parameter:

% degradation (TOC removal)

Value:

98.8

Sampling time:

3 wk

% Degradation 3

Parameter:

% degradation (test mat. analysis)

Value:

100

Sampling time:

3 wk

Remarks on result:

other: GC

Details on results:

According to the result of coulometer after 14 days, the oxygen absorbtion curve was changed. Therefore, the test was prolonged to 3 weeks. The result was calculated based on the value of sample 3 which showed trend of degradability.

Any other information on results incl. tables

Table 1. Test result of TOC

Sample	Height of peak (mm)			Apparent Concentrationµg/ml	Residual quantity (300 ml)
	test sub.	correction value by sludge	correction value by water
1. Sludge blank	5.4
3. Sludge + test sub.	5.6	0.2		1.0	0.3*
4. Sludge + test sub.	21.7	16.3	41.3	40.5	12.2
5. water + test sub.	44.2		34.3	101.0	30.3**
6. water + test sub.	37.2			85.0	25.5
water blank	2.9

* Result of this study was caluculated based on Sample 3.

** 5 was excluded because contamination was assumed.

Table 2. Result of GC determination

Sample	Mean of peak (µV sec)	Concentrationµg/ml	Residual quantity (30 ml)	Residue Mean (mg)	Degradation (%)
1. Sludge blank	0	0	0
3. Sludge + test sub.	0	0	0*	-	-
4. Sludge + test sub.	279977	820	24.6
5. water + test sub.	268997	790	23.7	24.9	100
6. water + test sub.	295530	870	26.1

* Result of this study was calculated based on Sample 3.

Applicant's summary and conclusion

Interpretation of results:

readily biodegradable