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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Disodium 4,4'-diaminostilbene-2,2'-disulphonate
EC Number:
230-847-3
EC Name:
Disodium 4,4'-diaminostilbene-2,2'-disulphonate
Cas Number:
7336-20-1
Molecular formula:
C14H14N2O6S2.2Na
IUPAC Name:
disodium 2,2'-ethene-1,2-diylbis(5-aminobenzenesulfonate)
Details on test material:
- Name of test material: 4,4-diamino-stilben-2,2-disulfonic acid, disodium salt
- Analytical purity: no data

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from BASF sewage works
- Concentration of sludge: 66,7 mg/L in the test
Duration of test (contact time):
53 d
Initial test substance concentration
Initial conc.:
400 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Solubilising agent: none used
- Test temperature: room temperature
- pH: 6.7 - 7.8
- pH adjusted: yes


TEST SYSTEM
- Culturing apparatus: all glass bottles (volume: 5L)
- Number of culture flasks/concentration: 1
- Measuring equipment: no data available
- Test performed in open system: no

SAMPLING
- Sampling frequency: after 3hours, 1, 4, 8, 15, 18, 27, 31, 36, 46 and 53 days

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
-1
Sampling time:
53 d

Any other information on results incl. tables

Degradation results
   0 h  3 h  1 d  4 d  8 d  15 d  18 d  27 d  31 d  36 d  46 d  53 d
 DOC measured [mg/l]  423  405  408  384  415  359  349  327  350  347  346  359
 TOC elimination [%]  -6  1  -1  -1  -7  7  8  12  5  4  2  -1
 pH  6.7 7.2    7.8  7.8 4.8  6.2  5.4  5.8    6.6  6.6

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed

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