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Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only secondary literature available

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
Chlortoluidine (3-Chlor-2-methylanilin, 5-Chlor-2-methylanilin, 3-Chlor-4-methylanilin)
Author:
Gesellschaft Deutscher Chemiker
Year:
1990
Bibliographic source:
Beratergremium für umweltrelevante Altstoffe (BUA), BUA-Stoffbericht 55
Reference Type:
publication
Title:
Do induced sperm-head abnormalities in mice specifically identify mammalian mutagens rather than carcinogens?
Author:
J.C. Topham
Year:
1980
Bibliographic source:
Mutat. Res. 74, pp. 379-387

Materials and methods

Principles of method if other than guideline:
Sperm abnormality test in mice. Application of the test substance intraperitoneally on 5 consecutive days. 5 weeks after last application analysis of sperm morphology.
GLP compliance:
not specified
Type of method:
in vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
5-chloro-o-toluidine
EC Number:
202-452-6
EC Name:
5-chloro-o-toluidine
Cas Number:
95-79-4
Molecular formula:
C7H8ClN
IUPAC Name:
5-chloro-2-methylaniline

Test animals

Species:
mouse
Strain:
other: (CB [male] x Balb/c [female])F1
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
corn oil
Details on exposure:
Application of 5mL/kg bw/day
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
5 consecutive days
Frequency of treatment:
daily
Duration of test:
5 weeks and 5 days
Doses / concentrations
Remarks:
Doses / Concentrations:
50, 100, 250, and 500 mg/kg bw/day
Basis:
no data
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
Five weeks after the last application the sperm was analyzed for abnormal sperm heads.

Results and discussion

Observed effects

In the dose group of 500 mg/kg bw/day all mice died.
No increase of the number of abnormal sperms were induced through the test substance in all other groups.

Applicant's summary and conclusion

Conclusions:
An application of the test substance intraperitoneally for 5 consecutive days did not induce a change in sperm morphology. At 500 mg/kg bw/day all mice died.