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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Under the conditions of this study, the test material attained 6 % degradation after 28 days and therefore cannot be considered to be readily biodegradable in accordance with EU criteria.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The key ready biodegradability study was conducted in line with GLP and in accordance with the standardised guidelines OECD 301B and EU Method C.4-C. It was assigned a reliability score of 1 in accordance with the criteria detailed by Klimisch (1997).

The test material, at a concentration of 10 mg Carbon/L, was exposed (in an aerobic aqueous medium) to activated sewage sludge micro-organisms with mineral medium in sealed culture vessels in the dark at 22 ± 2 °C for 28 days.

The degradation of the test material was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference material, sodium benzoate, together with a toxicity control were used for validation purposes.

Under the conditions of this study, the test material attained 6 % degradation after 28 days and therefore cannot be considered to be readily biodegradable in accordance with EU criteria.

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