Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-906-0 | CAS number: 89-41-8
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990 - 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-nitro-p-anisic acid
- EC Number:
- 201-906-0
- EC Name:
- 3-nitro-p-anisic acid
- Cas Number:
- 89-41-8
- Molecular formula:
- C8H7NO5
- IUPAC Name:
- 4-methoxy-3-nitrobenzoic acid
- Details on test material:
- purity: 98.2%
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, conventionial breeding
- Age at study initiation: about 3 - 5 months
- Weight at study initiation: 3.5 - 4.5 kg
- Housing: in air conditioned rooms in single cages
- Diet: Altromin 2123 - rabbit ( ad libitum) and 15 g hay per day
- Water: deionized and chlorinated water from automatic suspensors ( ad libitum)
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20+-3
- Humidity (%): 50+-20
- Photoperiod: 12 hrs dark / 12 hrs light
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- physiological saline
- Controls:
- no
- Amount / concentration applied:
- 500 mg suspended in 0.4 ml 0.9% NaCl-solution
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 72 hours
- Number of animals:
- 3 males
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm
- % coverage: 100%
- Type of wrap if used:plaster fixed with a semiocclusive bandage
REMOVAL OF TEST SUBSTANCE
- Washing: with slightly warm tap water
- Time after start of exposure: 4 hours
SCORING SYSTEM:
erythema: 0 - 4
edema: 0 - 4
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 60 min
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 60 min
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- no signs for irritation occured
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The substance is not skin irritating
- Executive summary:
The substance was tested for skin irritation according to OECD 404. 3 male New Zealand White rabbits got applicated 500 mg of the test substance suspended in 0.4 ml 0.9% NaCl-solution.The exposure time was 4 hours. After removing the plaster and washing the contaminated skin no sign for skin irritation could be observed
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
