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EC number: 920-684-2 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
This study showed that there were no toxic effects at saturation.
Key value for chemical safety assessment
Additional information
A study was performed to assess the effect of the test item on the growth of the green algaPseudokirchneriella subcapitata.The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009.
Pre-study solubility work conducted indicated that it was not possible to obtain a testable solution of the test item using traditional methods of preparation e.g. ultrasonication and high shear mixing.
A pre-study media preparation trial indicated that a dissolved test item concentration of approximately 0.43 mg/L was obtained from a saturated solution method of preparation indicating this to be the limit of water solubility of this item under test conditions.
Following a preliminary range-finding test,Pseudokirchneriella subcapitatawas exposed to a solution of the test item at 0-Hour measured concentration of 0.40 mg/L (six replicate flasks) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1°C. The test item solution was prepared by stirring an excess (100 mg/L) of test item in culture medium using a propeller stirrer at approximately 1500 rpm for 24 hours. After the stirring period any undissolved test item was removed by filtration (0.2 µm Sartorius Sartopore filter, first approximate 2 litres discarded in order to pre-condition the filter) to produce a saturated solution of the test item.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter® Multisizer Particle Counter.
Chemical analysis of the 100% v/v saturated solution at 0 hours showed a measured test concentration of 0.40 mg/L was obtained. A measured test concentration of 0.37 mg/L was obtained at 72 hours indicating that the test item was stable over the test period. Exposure of Pseudokirchneriella subcapitatato the test item gave EC₅₀values based on the 0-Hour measured test concentration of greater than 0.40 mg/L. The No Observed Effect Concentration was determined to be 0.40 mg/L.
This study showed that there were no toxic effects at saturation.
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