Registration Dossier
Registration Dossier
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EC number: 283-907-6 | CAS number: 84775-78-0 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Ascophyllum nodosum, Fucaceae.
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- June - September 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: in accordance with guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EPPO Guideline PP1/135(2)
- Deviations:
- no
- Principles of method if other than guideline:
- Study conducted in accordance with the Laboratory Internal Method BT189.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Italian Ministero della Salute
Test material
- Reference substance name:
- Ascophyllum nodosum, ext.
- EC Number:
- 283-907-6
- EC Name:
- Ascophyllum nodosum, ext.
- IUPAC Name:
- 283-907-6
- Details on test material:
- - Name of test material : ALGEAFERT SOLID K+
- Substance type: algal extract
- Physical state: solid, black micro-flakes
- pH water solution 1% : 9.2
- Water solubility 500 g/l at 25°C
- Lot/batch No.: 00680
- Expiration date of the lot/batch: March 09, 2012
- Storage condition of test material: in well ventilated areas, with containing device
Constituent 1
Test substrate
- Vehicle:
- no
Test organisms
open allclose all
- Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Species:
- Sinapis alba
- Plant group:
- Dicotyledonae (dicots)
- Species:
- Helianthus annuus
- Plant group:
- Dicotyledonae (dicots)
- Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Species:
- Avena sativa
- Plant group:
- Monocotyledonae (monocots)
- Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
Study design
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 29 d
- Remarks:
- in greenhouse
- Post exposure observation period:
- Effects evaluated at 7, 14 and 21 days after application
Test conditions
- Test temperature:
- range: 13.75-32.5 °C
- pH:
- pH value (0.01 M Ca Cl2) : 6.6 ± 0.5
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Biotecnologie BT
- Test container (type, material, size): pots
- Method of seeding: manual
- No. of seeds per container: 5, 3 or 2, depending upon the species.
- No. of plants: at least 20 for each concentration
- No. of replicates per treatment group: 4 (Avena sativa), 10 (Zea Mays), 7 for the other species.
- No. of replicates per control: 4 (Avena sativa), 10 (Zea Mays), 7 for the other species.
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: mid- Italy
- Pesticide use history at the collection site: none
- Soil texture: sandy loam
- Organic carbon (%): 0.97 ± 0.07
- Maximum water holding capacity (in % dry weigth): 35.1
- CEC: 9.0 ± 3.0
- Pretreatment of soil: none
GROWTH CONDITIONS
- Photoperiod: 16 h light and 8 h darkness
- Light source: artificial
- Light intensity and quality: 17800-20000 lux
- Relative humidity (%): 51.5 ± 86%
- Method of application: spraying with spray equipment with 2 nozzles at a spray pressure of 1.0 bar.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- visual assessment of seedling emergence and detrimental effects (chlorosis, necrosis, wilting leaf, stem deformations and mortality) at 7, 14 and 21 days after application
- measured endpoints: mortality (%) and final biomass (fresh shoot weight and shoot height).
VEHICLE CONTROL PERFORMED: no - Nominal and measured concentrations:
- 15 kg product /ha ( the test item dose rate being sprayed at 400 L water /ha on the selected species).
- Reference substance (positive control):
- yes
- Remarks:
- boric acid, tested on Cucumis sativus, parameter used 7-day EC50 for seedling shoot lenght
Results and discussion
Effect concentrationsopen allclose all
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: lenght (cm)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: -13.05 (v/s untreated)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: weight (g)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: -18.63 (v/s untreated)
- Species:
- Sinapis alba
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: lenght (cm)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: 1.29 (v/s untreated)
- Species:
- Sinapis alba
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: weight (g)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: - 10.26 (v/s untreated)
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: lenght (cm)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: -1.84 (v/s untreated)
- Species:
- Helianthus annuus
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: weight (g)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: 10.33 (v/s untreated)
- Species:
- Cucumis sativus
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: lenght (cm)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: 11.45 (v/s untreated)
- Species:
- Cucumis sativus
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: weight (g)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: 7.92 (v/s untreated)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: lenght (cm)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: 2.89 (v/s untreated)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: weight (g)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: 13.98 (v/s untreated)
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: lenght (cm)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: -5.94 (v/s untreated)
- Species:
- Zea mays
- Duration:
- 21 d
- Dose descriptor:
- other: % inhibition
- Effect conc.:
- ca. 15 kg/ha
- Nominal / measured:
- estimated
- Conc. based on:
- other: weight (g)
- Basis for effect:
- growth
- Remarks on result:
- other: RESULT: -12.06 (v/s untreated)
- Details on results:
- The t-student was used for statistical effect between concentrations and untreated.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- MORTALITY: EC50 value higher than 15 kg/ha. LENGHT: EC50 value higher than 15 kg/ha. WEIGHT: EC50 value higher than 15 kg/ha.
- Conclusions:
- The validity criteria of the test were satisfied. As for mortality, for all species the EC50 value resulted higher than 15 kg/ha. As for lenght, for all species the EC50 value resulted higher than 15 kg/ha. Brassica napus, Helianthus annuus and Zea mays increased lenght at the concentration tested compared with untreated. As for weight, for all species the EC50 value resulted higher than 15 kg/ha. Brassica napus, Sinapis alba and Zea mays increased weight at the concentration tested compared with untreated. Chlorosis, necrosis and other visual effects were not observed for any of the species.
- Executive summary:
The purpose of this study was to determine a possibleeffect ofAlgeafert Solid K+on seedling emergence and early growth of 6 non-target plants following exposure to the test substance applied to the soil.
Seeds were placed in contact with soil treated with the test substance and evaluated for effects after usually 7,14 and 21 days from the emergence of 50% of the seedlings in the control group. The substance was sprayed on the soil surface after the seeds had been planted. The measured endpoints are visual assessment of seedling emergence, fresh shoot weight and shoot height at 21 days after treatment and visual detrimental effects (chlorosis, mortality).
Treatment groups and one control group for four dicotyledonous species(Brassica napus, Sinapis alba,Helianthus annuus and Cucumis sativus) and two monocotyledonous species (Avena sativa and Zea mays) were set up in the test. Each group comprised at least twenty plants (monocotyledonous and dicotyledonous) for each concentration; the number of seeds planted (two, three or five) per pot depends upon the species.The control group was treated with deionised water. The following application rate was used in the limit test: 15 kg /ha
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