bis(2-ethylhexyl) 4,4’-{6-[4-tert-butylcarbamoyl)anilino]-1,3,5-triazine-2,4-diyldiimino}dibenzoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October-November 1997

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

421-450-8

EC Name:

-

Cas Number:

154702-15-5

Molecular formula:

C44H59N7O5

IUPAC Name:

2-ethylhexyl 4-[(4-{[4-(tert-butylcarbamoyl)phenyl]amino}-6-[(4-{[(2-ethylhexyl)oxy]carbonyl}phenyl)amino]-1,3,5-triazin-2-yl)amino]benzoate

Details on test material:

- Name of test material (as cited in study report): RA 3643
- Physical state: white powder
- Lot/batch No.: 87/96
- Storage condition of test material: stored in a refrigerator

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

The dilution water was DSWL, prepared from ground water.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Brachydanio rerio
- Source: M.B.Ruysbroek B.V. (Noordvliet 159, Maassluis)
- Length at study initiation (length definition, mean, range and SD): 1.95 +/- 0.11 cm
- Weight at study initiation (mean and range, SD): 0.06 +/- 0.01 cm
- Amount: 10 fish

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

202 mg/l

Test temperature:

24.9-25.6 °C

pH:

7.7-8.3

Dissolved oxygen:

The lowest value was 7.5 mg/l

Reference substance (positive control):

yes

Remarks:

0.01 ml/l DMSO

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2.48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Effect concentrations 3

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 2.48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Any other information on results incl. tables

Sublethal observations / clinical signs:

There was no mortality in the control medium. No adverse effects observed.

Applicant's summary and conclusion

Conclusions:

The test item is not acutely toxic to the zebra fish Brachydanio rerio, with respect to survival and condition, when exposed at its aqueous solubility level.