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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
Qualifier:
according to guideline
Guideline:
other: Commission Directive 92/69/EEC, Method C.4-D of July 31, 1992: Manometric Respirometry Test (EEC Publication No. L 383 A, December 1992)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Etilefrine
EC Number:
211-910-4
EC Name:
Etilefrine
Cas Number:
709-55-7
Molecular formula:
C10H15NO2
IUPAC Name:
3-[2-(ethylamino)-1-hydroxyethyl]phenol
Details on test material:
Name : Effortil-Rohbase
Batch No.: 310
Aggregate State at RT: solid
Colour: white
Purity: >98%
Analysis: meldting point
Certificate of Analysis: 19.07.2000
Sorage: in original container, at room temperature, in the dark

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
Species
activated sludge, micro organisms from a domestic waste water treatment plant

Origin
supplied by the sewage plant Groß-Zimmern, Germany

Conditioning
The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based an this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use.
Duration of test (contact time):
> 0 - <= 28 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System, Aqualytic, D-63263 Neu Isenburg, Germany) each day.
Reference substance
Reference substance:
aniline

Results and discussion

Preliminary study:
Biodegradation of Reference Item Aniline
The reference item Aniline was sufficiently degraded to 87% after 14 days, and to 101 % after 28 days of incubation. The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inocolum.
% Degradationopen allclose all
Parameter:
other: ThODNH4
Value:
> 34 - < 48
Sampling time:
10 d
Parameter:
other: ThODNH4
Value:
> 58 - < 62
Sampling time:
28 d
Parameter:
other: ThODN03
Value:
> 50 - < 53
Sampling time:
28 d
Details on results:
Biodegradation of Test Item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period degradation rates of 58 % and 62 %, based on ThODNH4, were found. At the end of the 10-day window biodegradation rates of 34 % and 48 % were determined. Thus, the percentage biodegradation did not exceed 60 % within the 10-day window.
If the calculation of biodegradation is based on ThODNO3, percentage biodegradation reached 50 % and 53 % after 28 days of incubation.


BOD5 / COD results

Results with reference substance:
Biodegradation in the Toxicity Control
In the toxicity control containing both, the test item and the reference item Aniline, 43 % biodegradation was noted within 14 days and 53 biodegradation was determined after 28 days of incubation.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not considered to be ready biodegradable
Executive summary:

Title:  Ready Biodegradability of Effortil-Rohbase in a Manometric Respirometry Test

Guidelines/Recommendations:  - Commission Directive 92/69/EEC, Method C.4-D of July 31,1992: Manometric Respirometry Test (EEC Publication No. L 383 A, December 1992)

- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992

- EPA Guideline 712-C-98-076: OPPTS 835.3110, "Ready Bio­degradability", January 1998.

Purpose: The test item Effortil-Rohbase was investigated for its ready bio­degradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

Results:

Biodegradation of Effortil-Rohbase: After correction of the mean biochemical oxygen demand of inoculum controls at the end of the 28-day exposure period degradation rates of 58 % and 62 %, based on ThODNH4, were found. At the end of the 10-day window biodegradation rates of 34 % and 48 % were determined. Thus, the percentage biodegradation did not exceed 60 % within the 10-day window. The test item can therefore not considered to be ready biodegradable. If the calculation of biodegradation is based on ThODNO3, percent­age biodegradation reached 50 % and 53 % after 28 days of incu­bation.

Biodegradation of Aniline: The reference item Aniline was sufficiently degraded to 87 % after 14 days, and to 101 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum

Biodegradation in the Toxicity Control: In the toxicity control containing both, the test item and the refer­ence item Aniline, 43 % biodegradation was noted within 14 days and 53 % biodegradation was determined after 28 days of incuba­tion. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.