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EC number: 434-800-1 | CAS number: 121776-33-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Phototransformation in soil
Administrative data
Link to relevant study record(s)
- Endpoint:
- phototransformation in soil
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EPA Guideline Subdivision N 161-3 (Photodegradation Studies on Soil)
- GLP compliance:
- yes
- Radiolabelling:
- yes
- Analytical monitoring:
- yes
- Remarks:
- TLC, HPLC, LSC
- Analytical method:
- high-performance liquid chromatography
- Details on sampling:
- Duplicate light exposed and dark samples, including volatile traps, were collected for analysis at 0, 24, 48, 72, 191,5, 479 hours after treatment (0, 1, 2, 3, 8 and 20 days).
- Details on soil:
- - soil type: Sable silty clay loam, obtained from Monmouth, IL, by Monsanto Company. This was the same type of soil used in the aerobic soil metabolism study.
- characteristics: 7% sand, 62% silt, 31% clay, 3.5% organic matter, pH: 5.8, field capacity (%) (1/3 bar): 29.5
- preparation: Soil was initially passed through a 2 mm sieve. Water was added to produce an aqueous slurry resulting in a thin even layer on the petri dish. Soil was then air dried. 686 µL of water was added just prior to application to achieve 75 % field capacity. - Light source:
- sunlight
- Light spectrum: wavelength in nm:
- >= 250 - <= 700
- Details on light source:
- - light source: natural sunlight, Richmond, California
- exposure start date: April 17,1990
- exposure termination date: May 7, 1990
- latitude: 37.45°N
- longitude: 122.26°W - Details on test conditions:
- - level of fortification: 0.41 lbs/acre based on soil surface area.
- light source: natural sunlight
- temperature range: light samples: 10.3 to 36.8°C (average 22.8+-3 .1°C), dark samples: 10.7 to 36.4°C (average 21.6+-1.6°C)
- dark control: Prepared and exposed concurrently in an identical apparatus with tight exposed samples, but were covered to prevent irradiation.
- sample intervals: Day 0, 1, 2, 3, 8 and Day 20
- volatile trapping: 1 Ethylene Glycol trap, 2 NaOH (10%) traps, continuous air flow.
- extraction methods: initial extraction: acetonitrile (5 mL), acetonitrile/water (1 :1, v/v) (5 mL), water (5 mL) (extracts were combined for analysis), additional base extraction (selected samples): 0.5N NaOH (3 d) 80°C.
- extraction efficiency: initial extraction: 78.9+-13.2% (light samples), 93.7+-5.2% (dark samples)
- analytical methods: Liquid Scintillation Counting, HPLC, Thin Layer Chromatography
- storage conditions: <0°C in freezer
- storage stability: Some samples were re-analyzed after 30 days of storage and no change in chromatographic distribution was noted - Duration:
- 20 d
- % Moisture:
- 75
- Temp.:
- 22.8 °C
- Initial conc. measured:
- 29.3 mg/kg soil d.w.
- Reference substance:
- not specified
- Dark controls:
- yes
- Preliminary study:
- Pilot exposures of [14C]MON 13900 indicated Mat the half-life would be less than 10 days thus the exposure times were planned to incorporate at least two half-lives rather than 30 days.
- DT50:
- 8.8 d
- Test condition:
- exposure to natural sunlight
- DT50:
- 1 435 d
- Test condition:
- in the dark
- Transformation products:
- yes
- No.:
- #1
- No.:
- #2
- Details on results:
- The photodegradation half-life of [14C]MON 13900 was calculated to be 8.8 days (211.3 hours) from a first-order plot. MON 13900 degraded slowly in the dark with an estimated half-life of 1435 days.
- Conclusions:
- The calculated half-life of photolysis for MON 13900 was 8.8 days using a linear first order kinetic model. Minimal degradation of MON 13900 occurred on the dark control soils during the study period, with an estimated half-life of 1435 days.
- Executive summary:
This study was carried out according to EPA Pesticide Assessment Guidelines, Subdivision N, Section 161 -3 to fulfill the data requirements for Photodegradation on Soil. The purpose of the study was to determine the reaction kinetics of photolysis of [14C]MON 13900 on Sable silty clay loam soil surfaces and if possible to characterize any significant photoproducts.
[14C]MON 13900 was exposed to natural sunlight on viable Sable silty clay loam soil for 20 days, concurrency with dark control samples. Samples were maintained in temperature controlled chambers with a constant humidified air flow and trapping of volatiles. The average temperatures throughout the study period were 22.8 +-3.1°C and 21.6 +-1.6 °C in the light and dark soil chambers, respectively. The nominal test substance concentration was 0.4 lbs/acre (based on soil surface area = 29.3 mg/kg soil d.w.). MON 13900 degraded rapidly on irradiated soils to a mixture of photoproducts (oxazoladine acid and several polar products which were readily extracted with acetonitrile and water), none of which exceeded 8.1 % of applied radiocarbon. 14CO2 represented 3.3% of applied radiocarbon at the end of the study. Soil-bound radiocarbon increased with time in the light exposed soil and required additional extraction with 0.5 N NaOH to be released. The calculated half-life of photolysis was 8.8 days using a linear first order kinetic model. Minimal degradation of MON 13900 occurred on the dark control soils during the study period, with an estimated half-life of 1435 days. All extracts were analysed by HPLC and selected samples were examined by TLC. Radiocarbon recoveries averaged 97.1+-5.3% and 98.7 +-5.3% for light exposed and dark control sample sets, respectively, throughout the study.
Reference
Average material balance and prodcut distribution expressed as a percent of applied radiocarbon
Day 20 | % MON 13900 | % MON 13900 oxazolidine acid | % unknowns | % Non-Extracted | % CO2 | Total recovery |
Light | 42.3 | 5.6 | 33.8 | 7.9 | 3.3 | 92.9 |
Dark | 94.4 | - | 0.2 | 7.8 | 0.7 | 103.1 |
This value is the sum of unidentified peaks detected in the organic and base extracts and in ethylene glycol traps (none exceeding 11.4% with evidence of more than one componen in this quantitated peak). MON 13900 degraded to several polar products which eluted at the solvent front in reverse phase HPLC. Analysis of base extracts of the soil using a Bio-Rad LC column for organic acids separatet at least 3 unknown peaks with early elution times. HPLC recoveries for the base extracts were consistently low (60 -70%) for both methods indicating the possibility of additional unknowns which were retained the columns.
Description of key information
This study was carried out according to EPA Pesticide Assessment Guidelines, Subdivision N, Section 161 -3 to fulfill the data requirements for Photodegradation on Soil. The purpose of the study was to determine the reaction kinetics of photolysis of [14C]MON 13900 on Sable silty clay loam soil surfaces and if possible to characterize any significant photoproducts.
[14C]MON 13900 was exposed to natural sunlight on viable Sable silty clay loam soil for 20 days, concurrency with dark control samples. Samples were maintained in temperature controlled chambers with a constant humidified air flow and trapping of volatiles. The average temperatures throughout the study period were 22.8 +-3.1°C and 21.6 +-1.6 °C in the light and dark soil chambers, respectively. The nominal test substance concentration was 0.4 lbs/acre (based on soil surface area = 29.3 mg/kg soil d.w.). MON 13900 degraded rapidly on irradiated soils to a mixture of photoproducts (oxazoladine acid and several polar products which were readily extracted with acetonitrile and water), none of which exceeded 8.1 % of applied radiocarbon. 14CO2 represented 3.3% of applied radiocarbon at the end of the study. Soil-bound radiocarbon increased with time in the light exposed soil and required additional extraction with 0.5 N NaOH to be released. The calculated half-life of photolysis was 8.8 days using a linear first order kinetic model. Minimal degradation of MON 13900 occurred on the dark control soils during the study period, with an estimated half-life of 1435 days. All extracts were analysed by HPLC and selected samples were examined by TLC. Radiocarbon recoveries averaged 97.1+-5.3% and 98.7 +-5.3% for light exposed and dark control sample sets, respectively, throughout the study.
Key value for chemical safety assessment
- Half-life in soil:
- 8.8 d
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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