Reaction mass of L-xylo-hex-2-ulosonic acid and ascorbic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-Keto-L-gulonsaure

IUPAC Name:

2-Keto-L-gulonsaure

Details on test material:

Physical state: white solid

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: The concentrations used were based on the results of a range finding study (LC50 after 96 hours between 100 and 1000 mg/l). Therefore the concentrations were spaced by a factor of 2.2 as follows: 0, 2340, 100, 220, 484, 1060, and 2340 mg/l.
- Sampling method: Randomization

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Strain:
- Source: Charles River Aquatics, Someren, Netherlands
- Age at study initiation (mean and range, SD): About 3 months
- Length at study initiation (length definition, mean, range and SD): 2.85 (2.6-3.2) cm
- Weight at study initiation (mean and range, SD): 0.24 (0.2 - 0.4)g
- Feeding during test: Tetra Min standard feed for aquarium fish ad libitum; and on workdays generally additionally live artemia

ACCLIMATION
- Acclimation period: test conditions correspond to housing conditions
- Type and amount of food: 1 day before and during exposure withdrawal of food

Study design

Test type:

semi-static

Total exposure duration:

96 h

Test conditions

Hardness:

About 2.5 mmol/l

Test temperature:

About 22 degrees Celsius

pH:

About 8.0

Dissolved oxygen:

>60%

Details on test conditions:

TEST SYSTEM
- Test vessel: all glass aquaria (30 x 22 x 24 cm)
- Aeration: Slight
- No. of organisms per vessel: 10

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Survival at 1, 4, 24, 48, 72, and 96 hrs

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 1 023.8 - <= 2 439.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Nominal Concentration (mg/l)	Number of fish	1 h	4 h	2 4h	48 h	72 h	96 h
0	10	0	0	0	0	0	0
2340 (pH adjusted)	10	0	0	0	0	0	0
100	10	0	0	0	0	0	0
220	10	0	0	0	0	0	0
484	10	0	0	0	0	0	0
1060	10	1	1	1	2	2	2
2340	10	10	10	10	10	10	10

Applicant's summary and conclusion

Conclusions:

The average between the analytically detected and nominal concentrations give a LC50 between 1023.8 and 2439.8 mg/l and a NOEC after 96 hours of 471.85 mg/l.

Executive summary:

Based on the nominal concentrations, the median lethal concentration (LC50) is between 1060 and 2340 mg/l. Mortality first occurred at a concentration of 1060 mg/l. The no observed effect concentration (NOEC) at 96 hours is 484 mg/l. The analytically detected concentrations gave a median LC50 of >1029.6 and 2390.8 mg/l and a NOEC of 460.6 mg/l.

Thus, the average between the analytically detected and nominal concentrations give a LC50 between 1023.8 and 2439.8 mg/l and a NOEC after 96 hours of 471.85 mg/l.