C14-16 (even numbered) and C16 (branched) saturated and unsaturated aliphatic hydrocarbons

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Nov-92

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report, acceptable with restrictions

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Cross-reference

Reason / purpose for cross-reference:

read-across: supporting information

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

Nov-92

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report, acceptable with restrictions

Justification for type of information:

A discussion and report on the read across strategy is given as an attachment in Section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Analytical monitoring:

yes

Details on sampling:

Samples were taken from the controls and each exposure concentration at 0 hours

Vehicle:

no

Details on test solutions:

1000mg 1-hexadecene was added to 1 litre water and stirred on three magnetic stirrers for 24 hours at test temperature (14°C) and then allowed to settle for 1 hour. The water accommodated fraction (WAF) was then withdrawn via a siphon prior to dilution to the required exposure concentrations.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Source: Westacre Trout Farm, Norfolk, UK

Holding conditions: The stock were obtained on 29th September 1992 and acclimatised to test conditions from the 3rd November 1992. Temperature remained in the range 14±1°C and dissolved oxygen within the range 10-10.1mg/l. The fish were fed daily to repletion with commercial trout pellet but food was not given in the 24 hour period immediately prior to exposure. No medication was given during the acclimatisation period, and no mortalities occurred in the 7 days prior to the test.

Life stage: At the end of the exposure period control fish had a mean length of 5.1cm (SD = 0.5cm) and a mean weight of 1.73g (SD = 0.51g).

Test type:

other: daily renewal

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

164±9 mgCaCO3/l

Test temperature:

13-14°C

pH:

7.7-8.1

Dissolved oxygen:

9.9-10mg/l

Salinity:

not applicable

Nominal and measured concentrations:

10, 18, 32, 56 and 100% WAF

Details on test conditions:

type and source of exposure medium laboratory tap water filtered, dechlorinated and softened to give a supply of water with the desired water hardness
test vessel 20litre glass aquaria
aeration yes
renewal rate daily
no of organisms per vessel 10
no of replicates per concentration 1
biomass loading 0.87g body weight/litre
photoperiod 16L:8D
light intensity data not reported
feeding none

Reference substance (positive control):

not required

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 other: mg/l WAF

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

1 000 other: mg/l WAF

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was seen in the controls or any test concentration during the exposure period.

Results with reference substance (positive control):

data not reported

Reported statistics and error estimates:

data not reported

Sublethal observations / clinical signs:

Values obtained at 0 hours demonstrated that Total Carbon (dissolved) values (TOC) for the exposure media were no higher than that of the control level. TOC values for the experimental medium were 21.0-22.5 mg/L vs 23.0 mg/L for the control. The actual concentrations in the test vessels were therefore negligible.

Validity criteria fulfilled:

yes

Conclusions:

The 96 hour NOELR is >1000mg/l WAF.

Executive summary:

This study follows standard guidelines and is carried out following GLP. The analytical method used was unable to distinguish any difference in TOC concentrations between the control and test solutions. Therefore the actual test concentrations (WAF) are negligible. No mortality was seen in the controls or any test concentration. Therefore, this study is interpreted as demonstrating that acute toxicity to fish is not observed at concentrations below the limit of solubility.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hexadec-1-ene

EC Number:

211-105-8

EC Name:

Hexadec-1-ene

Cas Number:

629-73-2

Molecular formula:

C16H32

IUPAC Name:

hexadec-1-ene

Details on test material:

100% purity, clear colourless liquid, stored in original container at room temperature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken from the controls and each exposure concentration at 0 hours

Test solutions

Vehicle:

no

Details on test solutions:

1000mg 1-hexadecene was added to 1 litre water and stirred on three magnetic stirrers for 24 hours at test temperature (14°C) and then allowed to settle for 1 hour. The water accommodated fraction (WAF) was then withdrawn via a siphon prior to dilution to the required exposure concentrations.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Source: Westacre Trout Farm, Norfolk, UK

Holding conditions: The stock were obtained on 29th September 1992 and acclimatised to test conditions from the 3rd November 1992. Temperature remained in the range 14±1°C and dissolved oxygen within the range 10-10.1mg/l. The fish were fed daily to repletion with commercial trout pellet but food was not given in the 24 hour period immediately prior to exposure. No medication was given during the acclimatisation period, and no mortalities occurred in the 7 days prior to the test.

Life stage: At the end of the exposure period control fish had a mean length of 5.1cm (SD = 0.5cm) and a mean weight of 1.73g (SD = 0.51g).

Study design

Test type:

other: daily renewal

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Test conditions

Hardness:

164±9 mgCaCO3/l

Test temperature:

13-14°C

pH:

7.7-8.1

Dissolved oxygen:

9.9-10mg/l

Salinity:

not applicable

Nominal and measured concentrations:

10, 18, 32, 56 and 100% WAF

Details on test conditions:

type and source of exposure medium laboratory tap water filtered, dechlorinated and softened to give a supply of water with the desired water hardness
test vessel 20litre glass aquaria
aeration yes
renewal rate daily
no of organisms per vessel 10
no of replicates per concentration 1
biomass loading 0.87g body weight/litre
photoperiod 16L:8D
light intensity data not reported
feeding none

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 other: mg/l WAF

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

1 000 other: mg/l WAF

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was seen in the controls or any test concentration during the exposure period.

Results with reference substance (positive control):

data not reported

Reported statistics and error estimates:

data not reported

Any other information on results incl. tables

Sublethal observations / clinical signs:

Values obtained at 0 hours demonstrated that Total Carbon (dissolved) values (TOC) for the exposure media were no higher than that of the control level. TOC values for the experimental medium were 21.0-22.5 mg/L vs 23.0 mg/L for the control. The actual concentrations in the test vessels were therefore negligible.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 hour NOELR is >1000mg/l WAF.

Executive summary:

This study follows standard guidelines and is carried out following GLP. The analytical method used was unable to distinguish any difference in TOC concentrations between the control and test solutions. Therefore the actual test concentrations (WAF) are negligible. No mortality was seen in the controls or any test concentration. Therefore, this study is interpreted as demonstrating that acute toxicity to fish is not observed at concentrations below the limit of solubility.