Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March to November 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Cross-reference
Reason / purpose:
read-across: supporting information
Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
March to November 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: This robust summary has a reliability rating of 1 because the study followed a standard guideline, followed GLP guidelines, and was conducted without deviations that would invalidate the study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
An equilabration study was conducted prior to the study to determine the length of time needed to develop water accommodated fractions (WAFs) at the 1000 mg/L loading level. The aqueous phase of the WAF was analyzed after 1, 24, 48, and 96 hours of stirring. In all samples, the quantity of test substance in solution was less than the limit of detection (0.5 mg/L). Further analysis of test substance in test media a loading level of 1 g/L also showed no detectable amounts of test substance. Consequently, analysis of treatment solutions from the definitive study were not conducted.
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
Organisms used in the test were from a laboratory culture were less than 24 hour old. The source of the culture was from ICI Brixham Laboratory from I.R.Ch.A., France.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
206 mg/L CaCO3
Test temperature:
18 to 19 degrees C
pH:
6.9 to 7.4
Dissolved oxygen:
9.0 to 9.1 mg/L
Salinity:
Reshwater medium
Nominal and measured concentrations:
The nominal loading rates were 10, 30, 100, 300, and 1000 mg/L. A control was also tested.
Details on test conditions:
The study was conducted in closed test systems that were not renewed during the study. The test systems used were 150 ml glass Erlenmeyer flasks. The control and treatment levels were evaluated in replicate test systems. Ten organisms, less than 24 hours old, were exposed in each of the control and treatment solution flasks and were not fed during the study.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no immobility reported in any of the treatment solutions and control at test termination.
Reported statistics and error estimates:
None
Validity criteria fulfilled:
yes
Conclusions:
The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.
Executive summary:

The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

Data source

Reference
Reference Type:
other: Study report; company data
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Hydrocarbons, C11-C14, containing n-alkanes, isoalkanes, cyclics with <2% aromatics

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
An equilabration study was conducted prior to the study to determine the length of time needed to develop water accommodated fractions (WAFs) at the 1000 mg/L loading level. The aqueous phase of the WAF was analyzed after 1, 24, 48, and 96 hours of stirring. In all samples, the quantity of test substance in solution was less than the limit of detection (0.5 mg/L). Further analysis of test substance in test media a loading level of 1 g/L also showed no detectable amounts of test substance. Consequently, analysis of treatment solutions from the definitive study were not conducted.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Organisms used in the test were from a laboratory culture were less than 24 hour old. The source of the culture was from ICI Brixham Laboratory from I.R.Ch.A., France.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None

Test conditions

Hardness:
206 mg/L CaCO3
Test temperature:
18 to 19 degrees C
pH:
6.9 to 7.4
Dissolved oxygen:
9.0 to 9.1 mg/L
Salinity:
Reshwater medium
Nominal and measured concentrations:
The nominal loading rates were 10, 30, 100, 300, and 1000 mg/L. A control was also tested.
Details on test conditions:
The study was conducted in closed test systems that were not renewed during the study. The test systems used were 150 ml glass Erlenmeyer flasks. The control and treatment levels were evaluated in replicate test systems. Ten organisms, less than 24 hours old, were exposed in each of the control and treatment solution flasks and were not fed during the study.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
LL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LL50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LL0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no immobility reported in any of the treatment solutions and control at test termination.
Reported statistics and error estimates:
None

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.
Executive summary:

The water accommodated fraction (WAF) of the test substance did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.