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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, non guideline, available as unpublished report, acceptable with restrictions
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in Section 13.
Cross-reference
Reason / purpose:
read-across: supporting information
Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, non guideline, available as unpublished report, acceptable with restrictions
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in Section 13.
Reason / purpose:
read-across source
Principles of method if other than guideline:
The acute and chronic toxicity of the test substance to earthworms was tested in a laboratory bioassay. Natural soil exposed in the field to the test substance was sampled over time. The bulk sample was analysed for physico chemical parameters and the concentration of the test substance. Earthworms were exposed to the soil samples and either their 14 day survival or 70 day effects on growth and reproducion recorded.
GLP compliance:
no
Remarks:
no data reported
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported
Analytical monitoring:
yes
Details on sampling:
A natural silty loam soil was dosed with drill cuttings containing the test substance in September 2001. Soil samples were taken after 2.5, 7, 8 and 10.5 months. 15 soil samples from 0-15cm depth were taken on each occassion, spread evenly across the test and control plots. The samples were bulked and homogenised.
Vehicle:
no
Details on preparation and application of test substrate:
Soil samples collected in the field determined the test concentrations. The bulk soil samples had measured corrected concentrations of 31428, 25195, 8145 and 10026ug/g dry weight over time. Only the concentration of 10026ug/g was tested for chronic effects on earthworms
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
large, healthy, adult worms were used
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
yes
Total exposure duration:
70 d
Post exposure observation period:
none
Test temperature:
22±2°C
pH:
6.5-7.7
Moisture:
remoistened
Details on test conditions:
three replicates of 500cc of remoistened soil were weighed out and placed in 900ml glass jarrs. The earthworms were added to the soil surface and the jars closed with a vented lid. The test was conducted under continual illumination.
Nominal and measured concentrations:
control and 10026ug/g dry weight (measured)
Reference substance (positive control):
no
Duration:
70 d
Dose descriptor:
NOEC
Effect conc.:
10 026 other: µg/g dry weight
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
70 d
Dose descriptor:
EC100
Effect conc.:
10 026 other: µg/g dry weight
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
70 d
Dose descriptor:
EC50
Effect conc.:
10 026 other: µg/g dry weight
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: approximate
Details on results:
No effects on mortality were seen at any test concentration
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Not applicable
Validity criteria fulfilled:
not specified
Remarks:
non standard test
Conclusions:
At the test concentration no effects on mortality were observed but reproduction was completely inhibited.
Executive summary:

At the test concentration no effects on mortality were observed but reproduction was completely inhibited. Although this test does not follow standard guidelines it is well described and includes analytical monitoring of the exposure concentrations. No effects on mortality were observed at the test concentration, however reproduction was completely inhibited. The NOEC reproduction cannot be calculated from this test, and this should be borne in mind whilst deriving the soil PNEC.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Principles of method if other than guideline:
The acute and chronic toxicity of the test substance to earthworms was tested in a laboratory bioassay. Natural soil exposed in the field to the test substance was sampled over time. The bulk sample was analysed for physico chemical parameters and the concentration of the test substance. Earthworms were exposed to the soil samples and either their 14 day survival or 70 day effects on growth and reproducion recorded.
GLP compliance:
no
Remarks:
no data reported

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Amodrill 1410, linear alpha olefin C14
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
A natural silty loam soil was dosed with drill cuttings containing the test substance in September 2001. Soil samples were taken after 2.5, 7, 8 and 10.5 months. 15 soil samples from 0-15cm depth were taken on each occassion, spread evenly across the test and control plots. The samples were bulked and homogenised.

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
Soil samples collected in the field determined the test concentrations. The bulk soil samples had measured corrected concentrations of 31428, 25195, 8145 and 10026ug/g dry weight over time. Only the concentration of 10026ug/g was tested for chronic effects on earthworms

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
large, healthy, adult worms were used

Study design

Study type:
laboratory study
Substrate type:
natural soil
Limit test:
yes
Total exposure duration:
70 d
Post exposure observation period:
none

Test conditions

Test temperature:
22±2°C
pH:
6.5-7.7
Moisture:
remoistened
Details on test conditions:
three replicates of 500cc of remoistened soil were weighed out and placed in 900ml glass jarrs. The earthworms were added to the soil surface and the jars closed with a vented lid. The test was conducted under continual illumination.
Nominal and measured concentrations:
control and 10026ug/g dry weight (measured)
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
70 d
Dose descriptor:
NOEC
Effect conc.:
10 026 other: µg/g dry weight
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
70 d
Dose descriptor:
EC100
Effect conc.:
10 026 other: µg/g dry weight
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
70 d
Dose descriptor:
EC50
Effect conc.:
10 026 other: µg/g dry weight
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: approximate
Details on results:
No effects on mortality were seen at any test concentration
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
non standard test
Conclusions:
At the test concentration no effects on mortality were observed but reproduction was completely inhibited.
Executive summary:

At the test concentration no effects on mortality were observed but reproduction was completely inhibited. Although this test does not follow standard guidelines it is well described and includes analytical monitoring of the exposure concentrations. No effects on mortality were observed at the test concentration, however reproduction was completely inhibited. The NOEC reproduction cannot be calculated from this test, and this should be borne in mind whilst deriving the soil PNEC.