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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline. Well conducted and documented study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Cross-referenceopen allclose all
Reason / purpose:
read-across: supporting information
Reference
Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline. Well conducted and documented study.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across source
Reason / purpose:
reference to same study
Qualifier:
no guideline available
Principles of method if other than guideline:
Acute contact test: the larvae are immersed in aqueous solutions of the test substance for 30 sec and mortality is assessed at 4h and finally at 24h.
GLP compliance:
not specified
Application method:
contact
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No test surrogate material
Analytical monitoring:
no
Details on sampling:
No sampling (no analytical monitoring)
Vehicle:
no
Details on preparation and application of test substrate:
- Method of test material application: whole body exposure to aqueous solution of the substance (immersion for 30 sec)
- Body part: whole body
- Controls: immersed in dilution water
Test organisms (species):
other: Epiphyas postvittana
Animal group:
other: Lepidoptera
Details on test organisms:
TEST ORGANISM
- Common name: Lightbrown apple moth (LBAM)
- Source: laboratory culture maintained since 1993, using the rearing procedure of Singh et al. (Singh, P., Calre, G.K., Ashby, M.D., 1985. Epihyas postvittana, in: "Handbook of Insect rearing II", Singh, P., Moore, R.F. (Eds.), Elsevier, New-York: 271-283).
- Age at test initiation (mean and range, SD): Larvae: 3rd (12 d) and 5th instar (21 d)

Study type:
laboratory study
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No post-exposure
Test temperature:
20 +/- 2°C
pH (if soil or dung study):
Not applicable
Humidity:
No data
Photoperiod and lighting:
Natural lighting and photoperiod
Details on test conditions:
TEST SYSTEM
- Test container / cage (material, size): 400 mm diameter fine wire mesh spherical cages, suspended in a 500-mL beaker of well-agitated emulsion for 30 sec, then larvae removed and placed into rearing media within a sealed plastic container
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per per control: 4


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality at 4h and 24 h


TEST CONCENTRATIONS
- 3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
- 5th instar: 0.5, 1.0, 2.5, 5, 10 mg/L
Nominal and measured concentrations:
Nominal concentrations:
3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
5th instar: 0.5, 1.0, 2.5, 5 and 10 mL/L
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
0.47 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar III
Remarks on result:
other: 95% CL: 0.36-0.58
Duration:
24 h
Dose descriptor:
other: LC90
Effect conc.:
0.97 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar III
Remarks on result:
other: 95% CL: 0.76-1.57
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
3.27 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar V
Remarks on result:
other: 2.64-3.94
Duration:
24 h
Dose descriptor:
other: LC90
Effect conc.:
11.68 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar V
Remarks on result:
other: 8.92-17.5
Details on results:
See table 1
Results with reference substance (positive control):
No reference substance
Reported statistics and error estimates:
See table 1

Table 1. Dose-mortality response (24 h exposure) ofEpiphyas postvittanato dipping in C15 Ampol CPD

 

Instar

Sample (n)

Slope (± SEM)

LC50(mL.L-1)

(95% CL)

LC90(mL.L-1)

(95% CL)

III

240

4.10 (± 0.55)

0.47 (0.36 - 0.58)

0.97 (0.76 - 1.57)

V

207

2.98 (± 0.37)

3.27 (2.64 - 3.94)

11.68 (8.92 - 17.5)

Validity criteria fulfilled:
not applicable
Conclusions:
The LC50 of C15 Ampol CPD for Epiphyas postvittana is 0.47 mL/L (i.e. 0.37 g/L)
Executive summary:

Lightbrown apple moth (Epihyas postvittana - LBMA) larvae (III and V instars) were exposed to C15 Ampol CPD (C15 alkane) by short immersion (30sec) into an aqueous emulsion of the substance. Mortality was assessed after 4h and 24 h.

The LC50 of C15 Ampol CPD for LBMA was found to be 0.47 mL/L ( i.e. 0.37 g/L).

Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Post-harvest disinfestation of lightbrown apple moth, Epiphyas postvittana Walker (Lepidoptera: Tortricidae), with an alkane
Author:
Taverner, Peter, Bailey, Peter, Hodgkinson, Mark, Beattie, G., Andrew C.
Year:
1999
Bibliographic source:
Pesticide Science 55: 1159-1166

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Acute contact test: the larvae are immersed in aqueous solutions of the test substance for 30 sec and mortality is assessed at 4h and finally at 24h.
GLP compliance:
not specified
Application method:
contact

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): C15 Ampol CPD
- Substance type: Petroleum product, UVCB
- Physical state: Liquid
- Analytical purity: 100% Commercial product
- Composition of test material, percentage of components: 100% paraffins (C15-alkanes)
- Density: 0.787 g/mL (at 15°C)


Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No test surrogate material

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
No sampling (no analytical monitoring)

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of test material application: whole body exposure to aqueous solution of the substance (immersion for 30 sec)
- Body part: whole body
- Controls: immersed in dilution water

Test organisms

Test organisms (species):
other: Epiphyas postvittana
Animal group:
other: Lepidoptera
Details on test organisms:
TEST ORGANISM
- Common name: Lightbrown apple moth (LBAM)
- Source: laboratory culture maintained since 1993, using the rearing procedure of Singh et al. (Singh, P., Calre, G.K., Ashby, M.D., 1985. Epihyas postvittana, in: "Handbook of Insect rearing II", Singh, P., Moore, R.F. (Eds.), Elsevier, New-York: 271-283).
- Age at test initiation (mean and range, SD): Larvae: 3rd (12 d) and 5th instar (21 d)

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No post-exposure

Test conditions

Test temperature:
20 +/- 2°C
pH (if soil or dung study):
Not applicable
Humidity:
No data
Photoperiod and lighting:
Natural lighting and photoperiod
Details on test conditions:
TEST SYSTEM
- Test container / cage (material, size): 400 mm diameter fine wire mesh spherical cages, suspended in a 500-mL beaker of well-agitated emulsion for 30 sec, then larvae removed and placed into rearing media within a sealed plastic container
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per per control: 4


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality at 4h and 24 h


TEST CONCENTRATIONS
- 3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
- 5th instar: 0.5, 1.0, 2.5, 5, 10 mg/L
Nominal and measured concentrations:
Nominal concentrations:
3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
5th instar: 0.5, 1.0, 2.5, 5 and 10 mL/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
0.47 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar III
Remarks on result:
other: 95% CL: 0.36-0.58
Duration:
24 h
Dose descriptor:
other: LC90
Effect conc.:
0.97 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar III
Remarks on result:
other: 95% CL: 0.76-1.57
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
3.27 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar V
Remarks on result:
other: 2.64-3.94
Duration:
24 h
Dose descriptor:
other: LC90
Effect conc.:
11.68 other: mL/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Instar V
Remarks on result:
other: 8.92-17.5
Details on results:
See table 1
Results with reference substance (positive control):
No reference substance
Reported statistics and error estimates:
See table 1

Any other information on results incl. tables

Table 1. Dose-mortality response (24 h exposure) ofEpiphyas postvittanato dipping in C15 Ampol CPD

 

Instar

Sample (n)

Slope (± SEM)

LC50(mL.L-1)

(95% CL)

LC90(mL.L-1)

(95% CL)

III

240

4.10 (± 0.55)

0.47 (0.36 - 0.58)

0.97 (0.76 - 1.57)

V

207

2.98 (± 0.37)

3.27 (2.64 - 3.94)

11.68 (8.92 - 17.5)

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The LC50 of C15 Ampol CPD for Epiphyas postvittana is 0.47 mL/L (i.e. 0.37 g/L)
Executive summary:

Lightbrown apple moth (Epihyas postvittana - LBMA) larvae (III and V instars) were exposed to C15 Ampol CPD (C15 alkane) by short immersion (30sec) into an aqueous emulsion of the substance. Mortality was assessed after 4h and 24 h.

The LC50 of C15 Ampol CPD for LBMA was found to be 0.47 mL/L ( i.e. 0.37 g/L).