Registration Dossier
Registration Dossier
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EC number: 700-762-0 | CAS number: -
Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline. Well conducted and documented study.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline. Well conducted and documented study.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Acute contact test: the larvae are immersed in aqueous solutions of the test substance for 30 sec and mortality is assessed at 4h and finally at 24h.
- GLP compliance:
- not specified
- Application method:
- contact
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No test surrogate material - Analytical monitoring:
- no
- Details on sampling:
- No sampling (no analytical monitoring)
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of test material application: whole body exposure to aqueous solution of the substance (immersion for 30 sec)
- Body part: whole body
- Controls: immersed in dilution water - Test organisms (species):
- other: Epiphyas postvittana
- Animal group:
- other: Lepidoptera
- Details on test organisms:
- TEST ORGANISM
- Common name: Lightbrown apple moth (LBAM)
- Source: laboratory culture maintained since 1993, using the rearing procedure of Singh et al. (Singh, P., Calre, G.K., Ashby, M.D., 1985. Epihyas postvittana, in: "Handbook of Insect rearing II", Singh, P., Moore, R.F. (Eds.), Elsevier, New-York: 271-283).
- Age at test initiation (mean and range, SD): Larvae: 3rd (12 d) and 5th instar (21 d)
- Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 24 h
- Post exposure observation period:
- No post-exposure
- Test temperature:
- 20 +/- 2°C
- pH (if soil or dung study):
- Not applicable
- Humidity:
- No data
- Photoperiod and lighting:
- Natural lighting and photoperiod
- Details on test conditions:
- TEST SYSTEM
- Test container / cage (material, size): 400 mm diameter fine wire mesh spherical cages, suspended in a 500-mL beaker of well-agitated emulsion for 30 sec, then larvae removed and placed into rearing media within a sealed plastic container
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per per control: 4
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality at 4h and 24 h
TEST CONCENTRATIONS
- 3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
- 5th instar: 0.5, 1.0, 2.5, 5, 10 mg/L - Nominal and measured concentrations:
- Nominal concentrations:
3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
5th instar: 0.5, 1.0, 2.5, 5 and 10 mL/L - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.47 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar III
- Remarks on result:
- other: 95% CL: 0.36-0.58
- Duration:
- 24 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 0.97 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar III
- Remarks on result:
- other: 95% CL: 0.76-1.57
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.27 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar V
- Remarks on result:
- other: 2.64-3.94
- Duration:
- 24 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 11.68 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar V
- Remarks on result:
- other: 8.92-17.5
- Details on results:
- See table 1
- Results with reference substance (positive control):
- No reference substance
- Reported statistics and error estimates:
- See table 1
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The LC50 of C15 Ampol CPD for Epiphyas postvittana is 0.47 mL/L (i.e. 0.37 g/L)
- Executive summary:
Lightbrown apple moth (Epihyas postvittana - LBMA) larvae (III and V instars) were exposed to C15 Ampol CPD (C15 alkane) by short immersion (30sec) into an aqueous emulsion of the substance. Mortality was assessed after 4h and 24 h.
The LC50 of C15 Ampol CPD for LBMA was found to be 0.47 mL/L ( i.e. 0.37 g/L).
Table 1. Dose-mortality response (24 h exposure) ofEpiphyas postvittanato dipping in C15 Ampol CPD
Instar |
Sample (n) |
Slope (± SEM) |
LC50(mL.L-1) (95% CL) |
LC90(mL.L-1) (95% CL) |
III |
240 |
4.10 (± 0.55) |
0.47 (0.36 - 0.58) |
0.97 (0.76 - 1.57) |
V |
207 |
2.98 (± 0.37) |
3.27 (2.64 - 3.94) |
11.68 (8.92 - 17.5) |
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Post-harvest disinfestation of lightbrown apple moth, Epiphyas postvittana Walker (Lepidoptera: Tortricidae), with an alkane
- Author:
- Taverner, Peter, Bailey, Peter, Hodgkinson, Mark, Beattie, G., Andrew C.
- Year:
- 1 999
- Bibliographic source:
- Pesticide Science 55: 1159-1166
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Acute contact test: the larvae are immersed in aqueous solutions of the test substance for 30 sec and mortality is assessed at 4h and finally at 24h.
- GLP compliance:
- not specified
- Application method:
- contact
Test material
- Reference substance name:
- C15 paraffins
- IUPAC Name:
- C15 paraffins
- Reference substance name:
- C15 Ampol CPD
- IUPAC Name:
- C15 Ampol CPD
- Details on test material:
- - Name of test material (as cited in study report): C15 Ampol CPD
- Substance type: Petroleum product, UVCB
- Physical state: Liquid
- Analytical purity: 100% Commercial product
- Composition of test material, percentage of components: 100% paraffins (C15-alkanes)
- Density: 0.787 g/mL (at 15°C)
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No test surrogate material
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No sampling (no analytical monitoring)
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of test material application: whole body exposure to aqueous solution of the substance (immersion for 30 sec)
- Body part: whole body
- Controls: immersed in dilution water
Test organisms
- Test organisms (species):
- other: Epiphyas postvittana
- Animal group:
- other: Lepidoptera
- Details on test organisms:
- TEST ORGANISM
- Common name: Lightbrown apple moth (LBAM)
- Source: laboratory culture maintained since 1993, using the rearing procedure of Singh et al. (Singh, P., Calre, G.K., Ashby, M.D., 1985. Epihyas postvittana, in: "Handbook of Insect rearing II", Singh, P., Moore, R.F. (Eds.), Elsevier, New-York: 271-283).
- Age at test initiation (mean and range, SD): Larvae: 3rd (12 d) and 5th instar (21 d)
Study design
- Study type:
- laboratory study
- Limit test:
- no
- Total exposure duration:
- 24 h
- Post exposure observation period:
- No post-exposure
Test conditions
- Test temperature:
- 20 +/- 2°C
- pH (if soil or dung study):
- Not applicable
- Humidity:
- No data
- Photoperiod and lighting:
- Natural lighting and photoperiod
- Details on test conditions:
- TEST SYSTEM
- Test container / cage (material, size): 400 mm diameter fine wire mesh spherical cages, suspended in a 500-mL beaker of well-agitated emulsion for 30 sec, then larvae removed and placed into rearing media within a sealed plastic container
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per per control: 4
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality at 4h and 24 h
TEST CONCENTRATIONS
- 3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
- 5th instar: 0.5, 1.0, 2.5, 5, 10 mg/L - Nominal and measured concentrations:
- Nominal concentrations:
3rd instar: 0.25, 0.5, 0.75, 1.0, 2.5 mL/L
5th instar: 0.5, 1.0, 2.5, 5 and 10 mL/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.47 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar III
- Remarks on result:
- other: 95% CL: 0.36-0.58
- Duration:
- 24 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 0.97 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar III
- Remarks on result:
- other: 95% CL: 0.76-1.57
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.27 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar V
- Remarks on result:
- other: 2.64-3.94
- Duration:
- 24 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 11.68 other: mL/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Instar V
- Remarks on result:
- other: 8.92-17.5
- Details on results:
- See table 1
- Results with reference substance (positive control):
- No reference substance
- Reported statistics and error estimates:
- See table 1
Any other information on results incl. tables
Table 1. Dose-mortality response (24 h exposure) ofEpiphyas postvittanato dipping in C15 Ampol CPD
Instar |
Sample (n) |
Slope (± SEM) |
LC50(mL.L-1) (95% CL) |
LC90(mL.L-1) (95% CL) |
III |
240 |
4.10 (± 0.55) |
0.47 (0.36 - 0.58) |
0.97 (0.76 - 1.57) |
V |
207 |
2.98 (± 0.37) |
3.27 (2.64 - 3.94) |
11.68 (8.92 - 17.5) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The LC50 of C15 Ampol CPD for Epiphyas postvittana is 0.47 mL/L (i.e. 0.37 g/L)
- Executive summary:
Lightbrown apple moth (Epihyas postvittana - LBMA) larvae (III and V instars) were exposed to C15 Ampol CPD (C15 alkane) by short immersion (30sec) into an aqueous emulsion of the substance. Mortality was assessed after 4h and 24 h.
The LC50 of C15 Ampol CPD for LBMA was found to be 0.47 mL/L ( i.e. 0.37 g/L).
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