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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
p-Nitrobenzoesäure-2-ethylhexylester
IUPAC Name:
p-Nitrobenzoesäure-2-ethylhexylester
Details on test material:
- Name of test material (as cited in study report): p-Nitrobenzoesäure-2-ethylhexylester
- Molecular formula (if other than submission substance): C15H21NO4
- Molecular weight (if other than submission substance): 279
- Substance type: chemical
- Physical state: liquid
- Analytical purity: approx. 99.9 %
- Lot/batch No.: 3299/95/146 Fr. 4A
- Storage condition of test material: room temperature
- Water solubility: 1.4 mg/L at 25 °C

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): taken from a laboratory STP feed with comunal and synthetic sewage water

- Concentration of sludge: 10 ml
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
< 10
Sampling time:
2 d
Parameter:
% degradation (O2 consumption)
Value:
20 - 30
Sampling time:
3 d
Parameter:
% degradation (O2 consumption)
Value:
60 - 70
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
80 - 90
Sampling time:
28 d

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable