Registration Dossier

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study in compliance with GLP-criteria.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid
EC Number:
608-403-9
Cas Number:
297132-04-8
Molecular formula:
C15H16O7
IUPAC Name:
4-({[4-(acryloyloxy)butoxy]carbonyl}oxy)benzoic acid
Test material form:
solid: crystalline
Details on test material:
- Name of test substance: 4-(4-Acryloyloxy-butoxycarbonyloxy)-benzoic acid
- Chemical name: no details
- Molecular formula: C15H16O7
- Aggregate state: solid, crystalline
- Colour: white
- Homogeneity: homogeneous
- Special conditions for storage: storage at room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Activated sludge from laboratory wastewater plants treating municipal sewage.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
ca. 35 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
other: TOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 2 °C

PRELIMINARY INVESTIGATIONS:
- TOC (mg/g): 594
- DOC (mg/g): 594
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
- Degradation degree of the reference substance after 14 days (% CO2/ThCO2): 70-80
- Degradation degree in the inhibition control after 14 days (% Co2/ThCO2): 70-80
- Physico-chemical (abiotic) elimination of the test substance (% DOC): <10 at the end of the test
- Elimination of the test substance by adsorption (% DOC): <10 after 5 days
% Degradation
Value:
80 - 90
Sampling time:
28 d
Details on results:
- Duration of the adaptation phase (days): 3
- Duration of the degradation phase (days): 22
- Degradation of the test substance at the end of the 10-day window (% DOC): 70-80
- Degradation degree of the test substance at the end of the test (% DOC): 80-90
- 10-day window criterion met: Yes

Any other information on results incl. tables

Degradation degree Dt% (CO2/ThCO2)

Day

RS

IH

PC

TS1

TS2

TSmean

0

0

0

-

0

0

0

1

4

0

4

2

3

2

4

9

8

10

9

3

15

22

43

9

26

4

96

72

48

49

27

5

37

35

38

31

35

6

46

45

46

41

44

7

51

51

52

49

51

8

55

56

59

56

58

9 60 60 64 61 63
10 64 64 68 65 67
 11 67 67 73 70 72
12 70  69    76  73  75 
13  72  71    79  75  77 
14  74  73    79  76  78 
15  75  75    78  76  78 
16  78  77    78  76 77 
17  79  78    78  76  77 
18  80  79    78  76  77 
19 78  80    78  76  77 
20   80 80    83  81  82 
21  82  81    87  83  85 
 22 84   81   90  84  87 
23  84  81    91  84  88 
24  84  81    92  84  88 
25  85  81    92  84  88 
26  85  81    92   84 88 
 27 85  81    92  84  88 
28  86  81    92  85  89 

RS: reference substance

IH: inhibition control

PC: abiotic control

TS: test substance

Applicant's summary and conclusion