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EC number: 940-543-9 | CAS number: 354-15-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The data source is the competent authority. Although details on test method and results are not completely described, the results are sufficient to estimate the biodegradation properties of the substance.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- 7 day-test, no data on water composition and inoculum.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,1,2-trichloro-1,2-difluoroethane
- EC Number:
- 940-543-9
- Cas Number:
- 354-15-4
- Molecular formula:
- C2HCl3F2
- IUPAC Name:
- 1,1,2-trichloro-1,2-difluoroethane
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): R122
- Molecular formula: CHCIF–ССI2F
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- not specified
- Duration of test (contact time):
- 7 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 9.36 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 18.72 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 37.44 mg/L
- Based on:
- act. ingr.
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Sampling time:
- 7 d
- Remarks on result:
- other: No significant effect of tested concentrations of HCFC 122a on biochemical oxygen consumption was observed.
Any other information on results incl. tables
Biochemical oxygen consumption.
HCFC 122a concentration (mg/l) |
Day 1 |
Day 3 |
Day 5 |
Day 7 |
|||||
mg/l O2 |
% in comparison with control |
mg/l O2 |
% in comparison with control |
mg/l O2 |
% in comparison with control |
mg/l O2 |
% in comparison with control |
||
Control |
1.2 |
100 |
2.4 |
100 |
2.6 |
100 |
2.9 |
100 |
|
9.36 |
1.19 |
99 |
2.35 |
98 |
2.57 |
99 |
2.81 |
95 |
|
18.72 |
1.16 |
97 |
2.33 |
97 |
2.5 |
96 |
2.76 |
95 |
|
37.44 |
1.14 |
95 |
2.31 |
96 |
2.47 |
95 |
2.73 |
94 |
|
The comparison between the amount of dissolved oxygen in the control water and in the experimental samples showed no significant effect of tested concentrations of HCFC 122a on biochemical oxygen consumption. The authors assumed that HCFC 122a were practically inert in terms of influence on the dynamics of biochemical oxygen consumption.
It can be concluded that no degration occurred under test conditions.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- No significant effect of tested concentrations of HCFC 122a on biochemical oxygen consumption was observerd. It can be concluded that no degradation occurred under test conditions.
- Executive summary:
The study was carried out to evaluate the influence of the substance on the dynamics of oxygen consumption in order to set the threshold concentrations and the approximate permissible levels in water. The test was conducted in accordance with the “Guidelines for the design and the scientific validation of the maximum allowable concentrations of harmful substances in water bodies” (1976).
HCFC 122a was added at three different concentrations to specially prepared water placed in bottles and the amount of oxygen consumption was analyzed on a period of 7 days. The biochemical oxygen consumption was determined on the 1st, 3rd, 5th, and 7th day.
The comparison between the amount of dissolved oxygen in the control water and in the experimental samples showed no significant effect of tested concentrations of HCFC 122a on biochemical oxygen consumption. The authors assumed that HCFC 122a were practically inert in terms of influence on the dynamics of biochemical oxygen consumption.
Basing on the results it can be concluded that no degration occurred under test conditions.
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