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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Nov 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
not relevant
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
17-Hydroxy-1,4,6-pregnatriene-3,20-dione
EC Number:
613-906-1
Cas Number:
66212-25-7
Molecular formula:
C21 H26 O3
IUPAC Name:
17-Hydroxy-1,4,6-pregnatriene-3,20-dione
Details on test material:
- Name of test material (as cited in study report): 1,4,6-Trienol

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 4 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Table 1: Cumulative immobilization

 Test compound    No. of Daphnia    No. of immobilized Daphnia  
       24 hours    48 hours  
 Control   20 0 0
 Saturated solution of ZK 5668   20 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A saturated solution of 1,4,6 Trienol with the concentration of 4.0 mg/I had no adverse effects on
the mobility of Daphnia. Therefore, the EC50/48 hours of ZK 5668 to Daphnia magna is higher than a saturated solution.
Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours)of 1,4,6 -Trienol to Daphnia magna in a limit-test. The study was conducted in agreement with the test guideline of the OECD, no. 202.

Twenty Daphnia were used tor the test solution of test substance and twenty tor the control group. The Daphnia were exposed to the test substance and the dilution water for a period of 48 hours under static conditions in a limit-test. Immobilization was recorded at 24 hours and 48 hours. In order to establish a saturated solution of this low soluble compound, a suspension of 1,4,6 -Trienol of 100 mg/l was prepared and stirred for 24 hours. Then it was filtered through a glass-fibre filter. This saturated solution obtained was used for the test. Sampies tor a TOC analysis were taken at 0 and 48 hours. The analytical results gave a concentration of 4.0 mg/l based on a calculation using the molecular formula of the test substance. No immobilized Daphnia was observed.