Copper(II) carbonate-copper(II) hydroxide (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, monitoring.

Data source

Reference

Reference Type:

publication

Title:

Influence of genetic and environmental factors on the tolerance of daphnia magna straus to essential and non-essential metals

Author:

Barata C et al.

Year:

1998

Bibliographic source:

Aquatic toxicol 42: 115-137

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1981

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Copper sulphate, pentahydrate

IUPAC Name:

Copper sulphate, pentahydrate

Constituent 2

Reference substance name:

7758-99-8

EC Number:

616-477-9

Cas Number:

7758-99-8

IUPAC Name:

7758-99-8

Details on test material:

- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Clone C, Clone F
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: For each genotype, two 2 L bulk cultures of 15 animais were maintained at the
desired water hardness with the addition of a standard organic extract (Baird et al. 1989). Animais were fed daily with Chlorella vulgaris Beijerinck (1 x 10E6 cells ml ). The culture medium was changed twice weekly and neonates removed within 24 h of release.
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Moderate-hard water: 90.7 mg/L CaCO3
Hard water: 179 mg/L CaCO3

Test temperature:

20±0.5°C

pH:

Moderate-hard water: 7.73±0.006
Hard water: 8.07±0.007

Dissolved oxygen:

near 100% of saturation

Details on test conditions:

TEST SYSTEM
- Test vessel:
- fill volume: 120 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic inorganic medium (APHA 1995)
- Total organic carbon: 0.41-0.48 mg/L
*for more details see study


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality observed by immobility


TEST CONCENTRATIONS
- Test concentration: 8-15

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.006 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Remarks:

CH2Cu2O5

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.003 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Cu

Basis for effect:

mortality

Any other information on results incl. tables

In this study the toxicity of copper to two different Daphnia magna clones (which are generally used in toxicity testing) were examined. Clone F was significantly more sensitive (LC50 48h = 3.2 µg/L Cu moderate hard water; LC50 48h = 23.1 µg/L Cu hard water) than clone C (LC50 48h = 15.4 µg/L Cu moderate hard water; LC50 48h = 119 µg/L Cu hard water).

LC50 48h = 3.2 µg/L Cu = 0.012 mg/L CuSO4*5H2O