4-amino-6-chlorotoluene-3-sulphonic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without analytical test item concentration verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EG-1 published by EPA, 1982

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4-chloro-5-methyl benzoic sulfonic acid sodium salt

IUPAC Name:

2-amino-4-chloro-5-methyl benzoic sulfonic acid sodium salt

Details on test material:

- Name of test material (as cited in study report): Chlor-p-Toluidinsaeure Na Salz
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: great waterflea
- Source: Originally obtained from the Institut National de Recherche Chimique Appliquee, France, since 1978 inhouse culture at BASF
- Age at study initiation (mean and range, SD): 6 - 24 h
- Method of breeding: Cultured in water with a total hardness of 2.5 ± 0.5 mM CaCO3, a Ca:Mg ratio of 4:1 and an oxygen content of > 2mg/L at a temperature of appr. 20 °C. 16:8 hours light:dark, light intensity appr. 5 µEinstein/(m2*s); wave length range from 400 - 750 nm
- Food type: brewers yeast and green algae suspension
- Frequency: brewers yeast at water exchange and green algae suspension daily, not on saturdays, sundays and bank holidays

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

3 mM (at test start)

Test temperature:

20 °C

pH:

7.86 - 8.02

Dissolved oxygen:

8.02 - 9.0 mg/L

Nominal and measured concentrations:

nominal: control, 62.5, 125, 250, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: centrifuge tubes with flat bottom
- Material, size, headspace, fill volume: 10 mL fill volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated charcoal filtered tap water, the acid constnat was adjusted to 4.3 using H2SO4, the total hardness was adjusted using distilled water
- Ca/Mg ratio: 4:1
- Conductivity: 650 µSiemens/cm (at test start)

OTHER TEST CONDITIONS
- Photoperiod: darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility