3,4-dimethoxyphenylacetonitrile

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study (OECD 405). Non-GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

Method: according to Fed. Reg. 38 (187), §1500.42, p. 27029, 27-Sep-1973

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

veratrylcyanid

IUPAC Name:

veratrylcyanid

Constituent 2

Reference substance name:

78/834

IUPAC Name:

78/834

Details on test material:

Veratrylcyanid; no data on purity given in the report

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

- diet: SSNIFF, Intermast

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye

Amount / concentration applied:

undiluted
Amount applied: 0.1 ml

Duration of treatment / exposure:

24 h

Observation period (in vivo):

72 h

Number of animals or in vitro replicates:

6 (1 female and 5 males).

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

other: animal #1 - #6

Time point:

other: 24 - 48 - 72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks on result:

other: individual scores: 0 - 0 - 0

Results 2

Irritation parameter:

iris score

Basis:

other: animal #1 -#6

Time point:

other: 24 - 48 - 72 h

Score:

0

Max. score:

2

Reversibility:

other: not applicable

Remarks on result:

other: individual scores: 0 - 0 - 0

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 24 - 48 - 72 h

Score:

1.7

Max. score:

3

Reversibility:

other: A full reversibility of the symptoms within 21 days can be expected.

Remarks on result:

other: individual scores: 2 - 2 - 1

Results 4

Irritation parameter:

chemosis score

Basis:

animal: animal #1 - #6

Time point:

other: 24 - 48 - 72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Remarks on result:

other: individual scores: 0 - 0 - 0

Results 5

Irritation parameter:

conjunctivae score

Basis:

other: animal #2, #4, #5 and #6

Time point:

other: 24 - 48 - 72 h

Score:

1

Max. score:

3

Reversibility:

other: A full reversibility of the symptoms within 21 days can be expected.

Remarks on result:

other: individual scores: 1 - 1 - 1

Results 6

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

other: 24 - 48 - 72 h

Score:

0.7

Max. score:

3

Reversibility:

fully reversible

Remarks on result:

other: individual scores: 1 - 1 - 0

Any other information on results incl. tables

Readings	Animal	Cornea		Iris	Conjunctiva			Additional findings
		op	ar		red	ch	di
24 h	1	0	0	0	2	0	1
	2	0	0	0	1	0	0
	3	0	0	0	1	0	0
	4	0	0	0	1	0	0
	5	0	0	0	1	0	1
	6	0	0	0	1	0	0
48 h	1	0	0	0	2	0	1
	2	0	0	0	1	0	0
	3	0	0	0	1	0	0
	4	0	0	0	1	0	0
	5	0	0	0	1	0	0
	6	0	0	0	1	0	0
72 h	1	0	0	0	1	0	0
	2	0	0	0	1	0	0
	3	0	0	0	0	0	0
	4	0	0	0	1	0	0
	5	0	0	0	1	0	0
	6	0	0	0	1	0	0
Mean 24-72 h	1-6	0	0	0	1.1	0	0.2

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information

Conclusions:

Based on the results of this study, the test substance vertarylcyanid does not need to be classified according to Regulation (EU) No. 1272/2008 and Directive 67/548/EEC.