Ethyl diphenylphosphinite

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN EN ISO 10712

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl diphenylphosphinite

EC Number:

211-951-8

EC Name:

Ethyl diphenylphosphinite

Cas Number:

719-80-2

Molecular formula:

C14H15OP

IUPAC Name:

ethyl diphenylphosphinite

Details on test material:

- Name of test substance (as cited in study report): Ethoxidiphenylphosphin dest.
- Batch No.: P. 96 0026
- State of aggregation: liquid
- Purity of test substance: 95.6 %
- Impurities: 0.8 % Diphenylphosphinsäureethylester, 3.6 % unknown
- Stability: not stable against oxygen and acids/caustic solutions
- Solubility in water: < 1 mg/L

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION OF THE STOCK SOLUTION AND DILUTIONS
- For the preparation of the stock solution (12500 mg/l) the test substance was stirred for about 20 hours at about 22°C (Ikamag RCT basic, step 8) in demineralized water.
- The undissolved test substance was removed by centrifugation of the stock solution (20 min at 7000g
(HERAEUS Cryofuge 6-6, 6000 rpm)). The test was performed with the supernatant (eluate).

The following nominal concentrations were made by diluting the aqueous extract with demineralized water:
10000 mg/l
5000 mg/l
2500 mg/I
1250 mg/l
625 mg/l
313 mg/l
156 mg/I
78.1 mg/l
39.1 mg/l

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

INOCULUM
- The test strain of Pseudomonas putida DSM 50026 used is obtained in regular intervais from DSM (German Collection of Microorganisms / Deutsche Sammlung von Mikroorganismen in Braunschweig) and is kept on agar slants for further cultivation in the Labor für Okotoxikologie of BASF AG Ludwigshafen.
- Pseudomonas putida is a gram-negative, aerobic, motile rod (length 2,0 to 4,0 pm , diameter 0,7 to 1,1 pm) with polar flagella. lt occurs ubiquitously in soil, surface water and waste-water. The optimal temperature for growth is among 25 and 30°C.

STEM CULTURE
Vessel: agar siant culture tubes (nominal volume 20 ml) plugged with gas permeable siliconesponge caps
Volume: 10 ml
Inoculation density: streak with inoculating loop
Incubation time: weekly passage
Temperature: 25°C ± 1°C

PRE CULTURE
Vessel: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable cellulose caps
Volume: 100 ml
Inoculation density: 10 FNU as optical density in Formazine Nephelometric Units
Incubation time: about 5 h with shaking
Temperature: 23 ± 1°C

TEST CULTURE
Vessel: tubes of glass with fiat bottom (nominal volume 50 ml) plugged with gas permeable siliconesponge caps
Volume: 10 ml
Inoculation density: 5 FNU
Incubation time: 16 ± 1 h with shaking
Temperature 23 ± 1°C

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

23 +/- 1°C

pH:

7.0 - 7.5

Details on test conditions:

TEST SYSTEM
- Replicates per test concentration: 4 inoculated replicates; 1 uninoculated replicate
- Replicates per control: 4 inoculated replicates; 1 uninoculated replicate

Results and discussion

Effect concentrations

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: bacterial cell multiplication

Details on results:

16 h EC50 > 10000 mg/L nominal