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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not yet available

Data source

Reference
Reference Type:
publication
Title:
Robust summary for 1,5,9-cyclododecatriene (revised).
Author:
DuPont Safety, Health & Environmental Excellence Center, Wilmington (Del., USA)
Year:
2003
Bibliographic source:
U.S. EPA, 46 pp

Materials and methods

Principles of method if other than guideline:
Method: other: Noakes DW and Sanderson DM (1969). Brit. J. Ind. Med. 26, 59-64.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Reference substance name:
1,5,9-Cyclododecatriene
IUPAC Name:
1,5,9-Cyclododecatriene
Constituent 2
Chemical structure
Reference substance name:
Cyclododeca-1,5,9-triene
EC Number:
225-533-8
EC Name:
Cyclododeca-1,5,9-triene
Cas Number:
4904-61-4
Molecular formula:
C12H18
IUPAC Name:
cyclododeca-1,5,9-triene
Details on test material:
1,5,9-Cyclododecatriene, purity not specified

Test animals

Species:
rat
Strain:
other: CD
Sex:
male/female
Details on test animals or test system and environmental conditions:
age: 12-13 weeks
- housed individually over the 24-hour exposure period, during which time they were deprived of food, but allowed water ad libitum. After exposur the rats were then housed 3/cage (genders separate)

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: no vehicle
Details on dermal exposure:
ADMINISTRATION:

- Occlusion: yes
- Doses: 2, 3, or 4 ml/kg bw
-test substance was placed onto the shorn dorso-lumbar skin, and bandaged to contact the skin using an impermeable dressing of aluminum foil and waterproof plaster.
- Removal of test substance: washing with a tepid dilute detergent  solution after 24 hours
Duration of exposure:
24 h
Doses:
2, 3, or 4 ml/kg bw
No. of animals per sex per dose:
2 rats of each sex per dose
Control animals:
no
Details on study design:
-observed for signs of intoxication during the following 9 days after exposure
Statistics:
no information

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 3 520 mg/kg bw
Mortality:
no mortalities
Clinical signs:
All animals were seen to have eschar at the application  site on removal of the occlusive dressing.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: other: Regulation EC 1272/2008
Executive summary:

Two rats of each sex (aged 12 -13 weeks) were used at each dose level (2, 3 or 4 mL/kg). The test substance was placed onto the shorn dorso-lumbar skin, and bandaged to contact the skin using an impermeable dressing of aluminium foil and waterproof plaster. The rats were housed individually over the 24 -hour exposure period, during which time they were deprived of food, but allowed water ad libitum. After 24 hours, the dressings were removed and the exposed area was washed with a tepid dilute detergent solution. The rats were then housed 3/cage, egnders separate, and observed for signs of intoxiciation during the following 9 days. None of the rabbits died during the test. All the rats had eschar on their backs when the occlusive dressing had been removed.

Under the conditions of this study the acute toxicity of 1,5,9-cyclododecatriene after dermal application in rabbits is very low with a LD50 higher than 3520 mg/kg (4 mL/kg).