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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation / corrosion
Remarks:
in vitro
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)
Deviations:
yes
Principles of method if other than guideline:
Standard protocol n°CEPC.B.ETU.PRO.EPI.04
Study of cytotoxicity on reconstituted epiderm by quantification of different endpoints (MTT, LDH, IL1a and IL8)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
(2S,3R,4R,5R,6R)-2-methyl-6-(pentyloxy)oxane-3,4,5-triol
EC Number:
700-713-3
Cas Number:
83161-22-2
Molecular formula:
C11H22O5
IUPAC Name:
(2S,3R,4R,5R,6R)-2-methyl-6-(pentyloxy)oxane-3,4,5-triol
Details on test material:
For technical reason (solubility), APRC5 was diluted at 30% in a water/glycerol (1/5) mixture
This mixture corresponds to the industrial form.

Test animals

Details on test animals or test system and environmental conditions:
test in vitro on reconstituted epiderm

Test system

Type of coverage:
other: in vitro test
Vehicle:
other: water/glycerol mixture
Controls:
yes
Amount / concentration applied:
APRC5 30%
Duration of treatment / exposure:
72h
Observation period:
72h
Details on study design:
test item : APRC5 30%
positive control item : Triton 4%

Results and discussion

In vivo

Results
Irritation parameter:
other: in vitro pamameters (LDH, IL1a, IL8)
Remarks on result:
other: slighly irritant
Irritant / corrosive response data:
 

Any other information on results incl. tables

 

   Test item  Positive control item
24h exposure  MTT % viability  89.9
24h exposure  LDH ratio

 1.4

 124.7
24h exposure  IL1a ratio  2.4  36
24h exposure  IL8 ratio  5.2 3.7 
72h exposure  MTT % viability  82.1  1.5
72h exposure  LDH ratio  1  77.3
72h exposure  IL1a ratio  2  7.2
72h exposure  IL8 ratio  4  0.9
 Classification    Slighly irritant  Irritant
     
       
       
       
       
       
       
       

Applicant's summary and conclusion

Conclusions:
slightly irritating