Pentyl 6-deoxy-alpha-L-mannopyranoside

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation / corrosion

Remarks:

in vitro

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)

Deviations:

yes

Principles of method if other than guideline:

Standard protocol n°CEPC.B.ETU.PRO.EPI.04
Study of cytotoxicity on reconstituted epiderm by quantification of different endpoints (MTT, LDH, IL1a and IL8)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

(2S,3R,4R,5R,6R)-2-methyl-6-(pentyloxy)oxane-3,4,5-triol

EC Number:

700-713-3

Cas Number:

83161-22-2

Molecular formula:

C11H22O5

IUPAC Name:

(2S,3R,4R,5R,6R)-2-methyl-6-(pentyloxy)oxane-3,4,5-triol

Details on test material:

For technical reason (solubility), APRC5 was diluted at 30% in a water/glycerol (1/5) mixture
This mixture corresponds to the industrial form.

Test animals

Details on test animals or test system and environmental conditions:

test in vitro on reconstituted epiderm

Test system

Type of coverage:

other: in vitro test

Vehicle:

other: water/glycerol mixture

Controls:

yes

Amount / concentration applied:

APRC5 30%

Duration of treatment / exposure:

72h

Observation period:

72h

Details on study design:

test item : APRC5 30%
positive control item : Triton 4%

Results and discussion

In vivo

Results

Irritation parameter:

other: in vitro pamameters (LDH, IL1a, IL8)

Remarks on result:

other: slighly irritant

Irritant / corrosive response data:

 

Any other information on results incl. tables

Test item  Positive control item 24h exposure  MTT % viability  89.9 3  24h exposure  LDH ratio  1.4  124.7 24h exposure  IL1a ratio  2.4  36 24h exposure  IL8 ratio  5.2 3.7  72h exposure  MTT % viability  82.1  1.5 72h exposure  LDH ratio  1  77.3 72h exposure  IL1a ratio  2  7.2 72h exposure  IL8 ratio  4  0.9  Classification    Slighly irritant  Irritant

Applicant's summary and conclusion

Conclusions:

slightly irritating