Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Acutely harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
31.6 mg/L

Additional information

No experimental data are available for 4,4'-diamino-3,3',5,5'-tetramethyldicyclohexylmethan (CAS 65962 -45 -0). Therefore a read-across approach to two structurally similar substances was performed.

The key study was performed on 2,2'-dimethyl-4,4'-methylenebis(cyclohexylamin) (CAS 6864-37-5) according to OECD 203 (NITE 2002) with Oryzias latipes. The 96-LC50 was determined to be 22.4 mg/L, the NOEC was 16 mg/L and the LC100 was 30 mg/L. The result is supported by a static-test study on CAS 6864-37-5 according to German industrial standard (DIN) 38412/15 (BASF 1988) performed with Leuciscus idus as test species. A 96-h LC50 of 31.6 mg/l was determined for non-neutralized samples (expressed as geometrical mean). The NOEC was determined to be 21.5 mg/l and the LC100 46.4 mg/l. Because DMD changes the pH in water, a neutralized sample with the highest test concentration (100 mg/l) was tested in addition. For this neutralized sample a diminution of the toxic effects was observed. 

An additional supporting study is a test performed on 4,4'-methylenebis-cyclohexanamine (CAS 1761-71-3) also performed according to German Industrial Standard DIN 38412, Part 15 using Leuciscus idus as test species (BASF AG, 1988; report no. 10F0562/875273). The 96 -h LC50 of 68.1 mg/L was determined for non-neutralised samples. A neutralised sample lead to a significant reduction of the mortality (96 -h LC50 > 100 mg/L).

In conclusion, the toxic potential for 4,4'-diamino-3,3',5,5'-tetramethyldicyclohexylmethan (CAS 65962 -45 -0) is assessed as acutely harmful to fish.