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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

Acutely harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
31.6 mg/L

Additional information

No experimental data are available for 4,4'-diamino-3,3',5,5'-tetramethyldicyclohexylmethan (CAS 65962 -45 -0). Therefore a read-across approach to two structurally similar substances was performed.

The key study was performed on 2,2'-dimethyl-4,4'-methylenebis(cyclohexylamin) (CAS 6864-37-5) according to OECD 203 (NITE 2002) with Oryzias latipes. The 96-LC50 was determined to be 22.4 mg/L, the NOEC was 16 mg/L and the LC100 was 30 mg/L. The result is supported by a static-test study on CAS 6864-37-5 according to German industrial standard (DIN) 38412/15 (BASF 1988) performed with Leuciscus idus as test species. A 96-h LC50 of 31.6 mg/l was determined for non-neutralized samples (expressed as geometrical mean). The NOEC was determined to be 21.5 mg/l and the LC100 46.4 mg/l. Because DMD changes the pH in water, a neutralized sample with the highest test concentration (100 mg/l) was tested in addition. For this neutralized sample a diminution of the toxic effects was observed. 

An additional supporting study is a test performed on 4,4'-methylenebis-cyclohexanamine (CAS 1761-71-3) also performed according to German Industrial Standard DIN 38412, Part 15 using Leuciscus idus as test species (BASF AG, 1988; report no. 10F0562/875273). The 96 -h LC50 of 68.1 mg/L was determined for non-neutralised samples. A neutralised sample lead to a significant reduction of the mortality (96 -h LC50 > 100 mg/L).

In conclusion, the toxic potential for 4,4'-diamino-3,3',5,5'-tetramethyldicyclohexylmethan (CAS 65962 -45 -0) is assessed as acutely harmful to fish.