Reaction mass of disodium [2,2'-(imino-kappaN)dibutanedioato-kappa2O1,O4(4-)]manganese(2-) and sodium sulphate

Short-term toxicity to aquatic invertebrates

Description of key information

Mn(II)-IDHA_OECD202_Daphnia magna: EC50 (48h) > 100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute toxicity of Mn(II)IDHA towards Daphnia magna was determined by OECD Guideline 202 in compliance with GLP (Konfederak, 2013). In this static test, the test organisms were exposed to the test item for 48 hours. The limit concentration and the control were split into eight replicates. There were five daphnids in each replicate. The daphnids were observed for immobilization after 24 and 48 hours of the exposure. Test organisms were considered immobile if they showed no ability to swim within 15 seconds after swirling the test vessel. The daphnids in the limit concentration of the test item (100.0 mg/L) and the control did not exhibit immobilization. Chemical analyses were performed using a validated method. Samples collected at the exposure initiation (t0) and at the exposure termination (t48) were analyzed. The average concentration of MnIDHA in samples collected at the exposure initiation (t0) was 88.7 % of the nominal concentration. The average concentration of MnIDHA in samples collected at the exposure termination (t48) was 91.6 % of the initial concentration. Since the concentration of MnIDHA was higher than 80% of the initial concentration at the exposure termination, the ECx values were determined on the basis of the nominal concentration of the test item. Immobilization was not observed in the limit test. In conclusion, the EC50 (48h) value is reported as > 100 mg/L. Due to the absence of immobilization at the limit concentration tested, the NOEC (48h) value can be indicated as ≥ 100 mg/L based on the nominal concentration.