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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

Mn(II)-IDHA_OECD201_Pseudokirchneriella subcapitata: ErC50 (72h) = 598 mg/L; NOEC (72h) < 10 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
598 mg/L
EC10 or NOEC for freshwater algae:
10 mg/L

Additional information

The impact of MnIDHA on the green algal growth was investigated during a 72-hour static toxicity study according to OECD Guideline 201 in compliance with GLP (Konfederak, 2013). The study was conducted in glass flasks with a capacity of 250 mL. Each replicate contained 100 mL. The initial density of the algae was 1 x 10 E4 cells/mL. The concentrations of the test item, MnIDHA of 10; 32; 100; 320 and 1000 mg/L and a control (0.0 mg/L) were used. Chemical analyses were performed using a validated method. Samples collected at the exposure initiation (t0) and at the exposure termination (t72) were analyzed. The average concentration of MnIDHA in samples collected at the exposure initiation (t0) was between 95.2 – 100.3% of the nominal concentration. The average concentration of MnIDHA in samples collected at the exposure termination (t72) was between 92.2 – 100.4% of the initial concentration. After 72 hours of the exposure, the algal cells were observed for changes in morphology. The cells in all test item concentrations did not differ from the control ones. Since the concentration of MnIDHA was in the range of 80 - 120% of the initial concentration at the exposure termination, the ECx values were determined on the basis of the nominal concentrations of the test item.

The concentration causing 50% inhibition of the growth rate of Pseudokirchneriella subcapitata, i.e. the ErC50 (72h) value is 597.57 mg/L. The ErC20 (72h) value is 140.86 mg/L, whereas the ErC10 (72h) value is 66.18 mg/L. For the growth rate, the LOEC (72h) is lower than or equal to 10 mg/L, whereas the NOEC (72h) is lower than 10 mg/L.

The mean concentration causing 50% inhibition of yield of Pseudokirchneriella subcapitata, i.e. the EyC50 (72h) value is 108.95 mg/L. The EyC20 (72h) value is 42.27 mg/L, whereas the EyC10 (72h) value is 25.76 mg/L. For yield, the LOEC (72h) is lower than or equal to 10 mg/L, whereas the NOEC (72h) is lower than 10 mg/L.