Ammonium oleate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1970-05-21- to 1970-06-01

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP study, similar to OECD Guideline. For justification of read across see section 13.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Oleic acid

EC Number:

204-007-1

EC Name:

Oleic acid

Cas Number:

112-80-1

Molecular formula:

C18H34O2

IUPAC Name:

octadec-9-enoic acid

Test material form:

other: liquid

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Fasting period before study: 24 hours
- Weight at study initiation: 200-300 g
- Housing: 5 animals per cage

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

4, 8, 16, 32, 48, 64 mL/kg, these values correlate to the following: 5262.125, 10524.25, 21048.5, 42097, 63145.5, 84194 mg/kg bw

No. of animals per sex per dose:

5 animals per dose including both male and female animals

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily observations
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD0

Effect level:

32 mL/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

47.3 mL/kg bw

Based on:

test mat.

95% CL:

> 40.9 - < 54.7

Remarks on result:

other: 47.3 mL/ kg bw can be converted to 42097 mg/kg bw using the densitiy of the substance (0.89 g/cm^3 = densitiy of CAS 112- 80-1)

Effect levels 3

Sex:

male/female

Dose descriptor:

LD100

Effect level:

64 mL/kg bw

Based on:

test mat.

Mortality:

Mortality occured from the 40 mL/kg bw dose group onwards. 1 animal of that dose group died on day 2, 3 animals of the 48 mL/kg bw dose group and all animals of the 64 mL/kg bw dose group died on day 1.

Clinical signs:

All dosage levels from 16 mL/kg and above had nasal hemorrhage, crustated occular areas, oozed urine and appeared to be debilitated prior to death.

Body weight:

no data

Gross pathology:

no data