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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From October 28th, 2002 to November 1st, 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1992
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Yellow 6314-PPT
IUPAC Name:
Yellow 6314-PPT
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Due to the low water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100 mg/l was continuously stirred at room temperature in the dark for 3 hours. Then the dispersion was filtered. Only the undiluted filtrate with the maximum concentration of dissolved test item was used as test medium. Additionally, a control was tested in parallel.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
20 daphnids tested per control
20 daphnids tested per undiluted filtrate

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
250.0 mg CaCO3 /l
Test temperature:
20 °C
pH:
7.8 control
7.7 - 7.9 undiluted filtrate
Dissolved oxygen:
8.3-8.8 mg/l control
8.3-8.4 mg/l undiluted filtrate
Nominal and measured concentrations:
Nominal 100 mg/l as loading rate
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The 48-hour NOEC and the 48-hour EC0 of the test item to Daphnia magna were determined to be at least at a loading rate of 100 mg/l. The 48-hour NOEC and the 48 hour EC0 might even be higher, but concentrations in excess of the loading rate of 100 mg/l have not been tested, due to the low water solubility of the test item in the used test water.

Applicant's summary and conclusion

Conclusions:
The test item had no toxic effect on the daphnia up to its solubility limit in test water.