2-butyryl-3-hydroxy-5-thiocyclohexan-3-yl-cyclohex-2-en-1-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

425-150-8

EC Name:

-

Cas Number:

94723-86-1

Molecular formula:

C15 H22 O3 S

IUPAC Name:

2-butanoyl-3-hydroxy-5-(thian-3-yl)cyclohex-2-en-1-one

Details on test material:

- Name of test material (as cited in study report): 625-Triketone
- Physical state: solid
- Analytical purity: 99.8% (area-%)
- Lot/batch No.:LJ 27 881/1

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Separately for each concentration 0.0469, 0.1015, 0.2190, 0.4701 and 1.00 g test substance were added to the test water using an ultra-turrax stirrer. Subsequently the fish were placed into the aquarla within 30 min after the test material was added.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Charles River Aquatics Someren, Netherlands
- Length at study initiation: 3.16 cm (range: 2.7 - 3.6)
- Weight at study initiation: 0.34 g (range: 0.2 - 0.5)
- Feeding during test
- Food type: Standard feed (TETRA MIN), on workdays generally additionally live artemia
- Amount: ad libitum
- Duration of housing: about 2 months

ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): none mortality during the last 14 days prior to test initiation
- Medical treatment: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmo/L

Test temperature:

23 °C

pH:

8.1 - 8.5

Dissolved oxygen:

8.2 - 9.2 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: control, 4.64, 10, 21.5, 46.4, 100 mg/L
- The analytically detected concentrations were initially in the range of 68.0% - 86.9% and after about 96 h between 90.5% - 103.3% of the nominal concentration.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.34 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water, not chlorinated, passed through a charcoal filter and aerated
- Alkalinity: about 5.5 mmol/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content after 1, 24, 48, 72, 96 h

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness

EFFECT PARAMETERS MEASURED: mortality and symptoms after 1, 4, 24, 48, 72, 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: yes
- Results used to determine the conditions for the definitive study: LC50 (96 h) between 10 and 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

4.64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Lethal effect concentrations based on measured concentrations:
LC0 (96 h) = 4.2 mg/L
LC50 (96 h) = 22 mg/L
LC100 (96 h) = 100.1 mg/L

- Detected symptoms: none
- Mortality of control: none

- Small amounts of test substance on the water surface and slight sedimentation was visible in all concentrations, increasing with the increase in the concentrations.