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Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information
In an Ames test , 3a,4,5,6,7,7a-hexahydro-4,7-methanoinden-6-yl acetate (tricyclodecenyl acetate), in dimethyl sulfoxide from doses 0.00001 to 0.01 ug/plate was not mutagenic in Salmonella typhimurium strains TA98, TA100,TA1535, TA1537, and TA1538 with and without addition of S9 liver fractions from Aroclor induced rats.
Link to relevant study records
Reference
Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
Data is from journal of Food and Chemical Toxicology
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay
Species / strain / cell type:
S. typhimurium, other: TA98, TA100, TA1535, TA1537, and TA1538
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9 liver fractions from Aroclor induced rats.
Test concentrations with justification for top dose:
0.00001 to 0.01 ug/plate
Vehicle / solvent:
dimethyl sulfoxide
Species / strain:
S. typhimurium, other: TA98, TA100, TA1535, TA1537, and TA1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.
Conclusions:
Interpretation of results (migrated information):
negative

3a,4,5,6,7,7a-hexahydro-4,7-methanoinden-6-yl acetate (tricyclodecenyl acetate) gives negative results for gene mutation in Ames test conductedon S. typhimurium strains TA98, TA100,TA1535, TA1537, and TA1538 without metabolic activation.
Executive summary:

In an Ames test (Ames et al., 1975), 3a,4,5,6,7,7a-hexahydro-4,7-methanoinden-6-yl acetate (tricyclodecenyl acetate), in dimethyl sulfoxide from doses 0.00001 to 0.01 ug/plate was not mutagenic in Salmonella typhimurium strains TA98, TA100,TA1535, TA1537, and TA1538 with and without addition of S9 liver fractions from Aroclor induced rats. Higher doses could not be tested due to bacteriostatic effects.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (negative)

Additional information

Additional information from genetic toxicity in vitro:

Chromosome aberration test for 3a,4,5,6,7,7a-hexahydro-4,7-methanoinden-6-yl acetate in chinese hamster Ovary (CHO) estimates negative result by using Multicase model A61.

Also the estimated mutations results in Danish QSAR for mammalian cells (mouse lymphoma) with 3a,4,5,6,7,7a- hexahydro-4,7- methanoinden-6-yl acetate were observed to be negative results.

Justification for classification or non-classification

Substance will not be classified in Genetic toxicity category as per the C & L criteria set by EU.

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