reaction mass of: tetrasodium 7-(4-(4-fluoro-6-(4-(2-sulfonatoethylsulfonyl)phenylamino)-1,3,5-triazin-2-ylamino)-2-ureidophenylazo)naphthalene-1,3,6-trisulfonate;tetrasodium 7-(4-(4-hydroxy-6-(4-(2-sulfonatoethylsulfonyl)phenylamino)-1,3,5-triazin-2-ylamino)-2-ureidophenylazo)naphthalene-1,3,6-trisulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 February 1998 to 12 February 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted to recent EU & OECD test guidance in compliance with GLP

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Analytical monitoring:

no

Details on sampling:

- Concentrations: 100 mg/L

Vehicle:

yes

Details on test solutions:

The test substance was weighed into a beaker, mixed with water for dilution and homogenized with the Ultra-Turrax.

In order to determine the substance concentration, water samples were taken at study start and study end from the 100 mg test concentration and the control.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Clone 5 (IRChA, France)
- Source: Stock culture of Hoechst Marion Roussel Deutschland GmbH
- Method of breeding: 100 mL beakers (height 90 mm, diameter 50 mm), filled with 70 mL test water (according to Elendt, M4) served as culture vessels. One daphnid was used per culture vessel. The animals were used for breeding up to an age of 42 days.
- Feeding during test: No

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No post exposure - 48 hour study

Hardness:

2.1 - 2.3 mmol Ca2+ + Mg2+ / L

Test temperature:

20.5-20.8° C

pH:

7.7-7.9

Dissolved oxygen:

8.7-9.0 mg/L

Salinity:

Not applicable.

Nominal and measured concentrations:

100 mg/L (nominal)

Details on test conditions:

The study was conducted in a static system. The test chambers were 250 mL beakers (height 110 mm, diameter 70 mm, water level about 75 mm), each filled with 200 mL test water. The room temperature was about 21⁰C. The room was illuminated by fluorescent tubes in a day/night cycle, with each period lasting 12 hours.

After the test concentrations had been prepared and water parameters were recorded, 10 daphnids were used in each test chamber. The daphnids received no feed for the entire study period. Inspection of the daphnids took place after 24 and 48 hours and involved recording the immobility. Immobile daphnids were removed from the chambers. Those animals not able to swim within 15 seconds after gentle agitation of the test container were considered to be immobile. The water parameters were measured after adding the substance and at the end of the test.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In this 48-hour acute toxicity study of Daphnia magna (water flea) no immobility was observed in the 100 mg/L-group and in the control group.

Results with reference substance (positive control):

Not applicable.

Reported statistics and error estimates:

Not measured.

	after 24 hours	after 48 hours
ECO (mg/L)	100	100
EC50 (mg/L)	>100	>100
EC 100 (mg/L)	not determined	not determined

Validity criteria fulfilled:

yes

Conclusions:

In this 48-hour acute toxicity study of the substance in Daphnia magna (water flea) no immobility was observed in the 100 mg/L-group and in the control group.

Under the conditions of this test the EC50 of the substance after 48 hours was > 100 mg/L.

Executive summary:

Study conducted to EU test guidance 92/69/EEC part C2 and OECD test guideline 202 in compliance witth GLP.

Under the conditions of the test the EC50 > 100 mg/L. The substance is not considered to pose a hazard to Daphnia Magna.

Description of key information

Under the conditions of the test the EC50 > 100 mg/L. The substance is not considered to pose a hazard to Daphnia Magna

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

A study was conducted according to EU test guidance 92/69/EEC part C2 and OECD test guideline 202 in compliance with GLP.

Under the conditions of the test the EC50 > 100 mg/L. The substance is not considered to pose a hazard to Daphnia Magna