Decanoic acid, mixed esters with dipentaerythritol, heptanoic acid and octanoic acid.

Endpoint summary

Description of key information

The read across for substance, CAS: 70851-04-6; EC: 453-480-2; is based upon the analogous substances to which basic form, degree of substitution  of functional groups is not considered to effect the proposed read across for the endpoint of skin irritation/corrosion. Based on the information available for the read across substances, the substance is expected to be irritating or corrosive to skin.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Reference 1

Endpoint:

skin irritation: in vitro / ex vivo

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

an in vitro skin irritation study does not need to be conducted because adequate data from an in vivo skin irritation study are available

Reference 2

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Composition of test substance unclear.

Principles of method if other than guideline:

Application of 0.5 mL to an area of approx. 2.5 cm x 2.5 cm.

GLP compliance:

not specified

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

- Weight at study initiation: 3.3 - 4.0 kg

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL
- Concentration (if solution): pure compound (containing up to 2% of an additive package which was not further specified by the sponsor)

Duration of treatment / exposure:

4 h

Observation period:

12 d
Reading time points: 30 - 60 min, 24, 48, 72, 96 h, 7, 8 and 12 d

Number of animals:

3 males

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm² on the shorn left flank (dorso-lumbar region)

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with warm water upon the removal of the dressing
- Time after start of exposure: 4 h

SCORING SYSTEM: Draize scoring system

Irritation parameter:

erythema score

Basis:

animal: #15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

2.7

Max. score:

4

Reversibility:

fully reversible within: 12 d

Irritation parameter:

erythema score

Basis:

animal: #16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 8 d

Irritation parameter:

erythema score

Basis:

animal: #17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 8 d

Irritation parameter:

edema score

Basis:

animal: #15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

3

Max. score:

4

Reversibility:

fully reversible within: 4 d

Irritation parameter:

edema score

Basis:

animal: #16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

3.7

Max. score:

4

Reversibility:

fully reversible within: 8 d

Irritation parameter:

edema score

Basis:

animal: #17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

3.7

Max. score:

4

Reversibility:

fully reversible within: 8 d

Irritant / corrosive response data:

Following the single 4-hour application, moderate to severe erythema and moderate to severe edema were observed for 2 or 3 days after application in all animals. This regressed and no signs of erythema or edema were evident 4 to 12 days after application. Thickening of the skin at the application site was seen on one animal between 4 and 7 days after application. No other signs of irritations were observed.

Other effects:

Yellow staining by the test sample was observed at the application site of one animal one day after application but this did not obscure the assessment of any erythema that was present.

Table 1: Erythema score

Animal Number	1 h	24 h	48 h	72 h	4 days	7 days	8 days	12 days	Mean Score (24 -72 h)
15	4	3	3	2	2	1		0	1.0
16	3	3	2	1	1		0		1.3
17	4	3	2	1	1		0		1.3

 

Table 2: Edema Score

Animal Number	1 h	24 h	48 h	72 h	4 days	7 days	8 days	12 days	Mean Score (24 -72 h)
15	4	3	4	2	0	0	-	0	3.0
16	4	4	4	3	3	-	0	-	3.7
17	4	4	4	3	3	-	0	-	3.7

Interpretation of results:

other: inconclusive

Remarks:

Criteria used for interpretation of results: EU

Reference 3

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Composition of test substance unclear.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

Lack of details in study documentation

GLP compliance:

not specified

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 3.5 - 3.8 g

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL

Duration of treatment / exposure:

4 h

Observation period:

12 d
Reading time points: 30 - 60 min, 24, 48, 72 h, 7, 11 and 12 d

Number of animals:

3 males

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm² on the shorn left flank (dorso-lumbar region)

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with warm water upon the removal of the dressing
- Time after start of exposure: 4 h

SCORING SYSTEM: Draize scoring system

Irritation parameter:

erythema score

Basis:

animal: #15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 12 d

Irritation parameter:

erythema score

Basis:

animal: #16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 11 d

Irritation parameter:

erythema score

Basis:

animal: #17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 7 d

Irritation parameter:

edema score

Basis:

animal: #15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 7 d

Irritation parameter:

edema score

Basis:

animal: #16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 7 d

Irritation parameter:

edema score

Basis:

animal: #17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 7 d

Irritant / corrosive response data:

Following the single 4-hour application, very slight to well-defined erythema and very slight to slight oedema were observed in all animals. This regressed and no signs of erythema or oedema were evident 11 or 12 days after application. Slight desquamation at the application site of one animal was observed 7 days after application.

Table 1: Erythema score

Animal Number	1 h	24 h	48 h	72 h	7 days	11 days	12 days	Mean Score (24 -72 h)
15	2	-	1	1	1	-	0	1.0
16	2	2	1	1	1	0	-	1.3
17	1	2	1	1	0	-	-	1.3

 

Table 2:Edema Score

Animal Number	1 h	24 h	48 h	72 h	7 days	11 days	12 days	Mean Score (24 - 72 h)
15	1	-	1	1	0	-	0	1.0
16	2	2	2	1	0	0	-	1.7
17	1	2	2	1	0	-	-	1.7

Interpretation of results:

other: inconclusive

Remarks:

Criteria used for interpretation of results: EU

Reference 4

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. (Limited documentation but relevant data given)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

limited documentation but relevant data given

GLP compliance:

not specified

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 5.0 - 5.2 g

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL

Duration of treatment / exposure:

4 h

Observation period:

4 d
Reading time points: 30 - 60 min, 24, 48, 72 and 96 h

Number of animals:

3 (females)

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): cleansing with clean warm water
- Time after start of exposure: 4 h (upon removal of the dressing)

SCORING SYSTEM: Draize scoring system

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 4 d

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 4 d

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 4 d

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48 h

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48 h

Irritation parameter:

edema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48 h

Table 1: Erythema scores

Animal Number	24 hours	48 hours	72 hours	96 hours	Mean Score
26	2	1	1	0	1.3
35	2	2	1	0	1.7
36	2	1	1	0	1.3

 

Table 2: Edema Score

Animal Number	24 hours	48 hours	72 hours	96 hours	Mean Score
26	1	0	0	0	0.3
35	1	0	0	0	0.3
36	1	0	0	0	0.3

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

CLP: not classified
DSD: not classified

Reference 5

Endpoint:

skin irritation: in vivo

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

1 substance available for read-across

Adequacy of study:

other information

Justification for type of information:

see the attached justification in Section 13 for full details.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Irritation parameter:

erythema score

Basis:

animal: #15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

2.7

Max. score:

4

Reversibility:

fully reversible within: 12 d

Remarks on result:

other: CAS 68424-31-7 (1991b)

Irritation parameter:

erythema score

Basis:

animal: #16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 8 d

Remarks on result:

other: CAS 68424-31-7 (1991b)

Irritation parameter:

erythema score

Basis:

animal: #17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible within: 8 d

Remarks on result:

other: CAS 68424-31-7 (1991b)

Irritation parameter:

edema score

Basis:

animal: #15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

3

Max. score:

4

Reversibility:

fully reversible within: 4 d

Remarks on result:

other: CAS 68424-31-7 (1991b)

Irritation parameter:

edema score

Basis:

animal: #16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

3.7

Max. score:

4

Reversibility:

fully reversible within: 8 d

Remarks on result:

other: CAS 68424-31-7 (1991b)

Irritation parameter:

edema score

Basis:

animal: #17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

3.7

Max. score:

4

Reversibility:

fully reversible within: 8 d

Remarks on result:

other: CAS 68424-31-7 (1991b)

Irritation parameter:

erythema score

Basis:

animal: 15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 12 d

Remarks on result:

other: CAS 68424-31-7 (1991c)

Irritation parameter:

erythema score

Basis:

animal: 16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 11 d

Remarks on result:

other: CAS 68424-31-7 (1991c)

Irritation parameter:

erythema score

Basis:

animal: 17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 7 d

Remarks on result:

other: CAS 68424-31-7 (1991c)

Irritation parameter:

edema score

Basis:

animal: 15

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 7 d

Remarks on result:

other: CAS 68424-31-7 (1991c)

Irritation parameter:

edema score

Basis:

animal: 16

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 7 d

Remarks on result:

other: CAS 68242-31-7 (1991c)

Irritation parameter:

edema score

Basis:

animal: 17

Time point:

other: mean over 24 h, 48 h and 72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 7d

Remarks on result:

other: CAS 68424-31-7 (1991c)

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 4d

Remarks on result:

other: CAS 68424-31-7 (1991a)

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 4d

Remarks on result:

other: CAS 68424-31-7 (1991a)

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1.3

Max. score:

4

Reversibility:

fully reversible within: 4d

Remarks on result:

other: CAS 68424-31-7 (1991a)

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48h

Remarks on result:

other: CAS 68424-31-7 (1991a)

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48h

Remarks on result:

other: CAS 68424-31-7 (1991a)

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible within: 48h

Remarks on result:

other: CAS 68424-31-7 (1991a)

Interpretation of results:

GHS criteria not met

Conclusions:

The read across for substance, CAS: 70851-04-6; EC: 453-480-2; is based upon the analogous substances to which basic form, degree of substitution of functional groups is not considered to effect the proposed read across for the endpoint of skin irritation/corrosion. Based on the information available for the read across substances, the substance is expected to be irritating or corrosive to skin.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Reference 1

Endpoint:

eye irritation: in vitro / ex vivo

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

an in vitro eye irritation study does not need to be conducted because adequate data from an in vivo eye irritation study are available

Reference 2

Endpoint:

eye irritation: in vivo

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

1 substance available for read-across

Adequacy of study:

weight of evidence

Justification for type of information:

see justification attached in Section 13 for full details

Reason / purpose for cross-reference:

read-across source

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility not applicable

Remarks on result:

other: CAS 68424-31-7

Irritation parameter:

iris score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

other: reversibility not applicable

Remarks on result:

other: CAS 68424-31-7

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

3

Reversibility:

other: reversibility not applicable

Remarks on result:

other: CAS 68424-31-7

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility not applicable

Remarks on result:

other: CAS 68424-31-7

Interpretation of results:

GHS criteria not met

Conclusions:

The read across for substance, CAS: 70851-04-6; EC: 453-480-2; is based upon the analogous substances to which basic form, degree of substitution of functional groups is not considered to effect the proposed read across for the endpoint of eye irritation. Based on the information available for the read across substances, the substance is expected to be not irritating to eyes.

Reference 3

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (lack of data on test substance and animal husbandry).

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

(lack of details on test substance and animal husbandry)

GLP compliance:

not specified

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 3.6 - 4.6 kg

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL

Duration of treatment / exposure:

single application without washing

Observation period (in vivo):

3 d

Number of animals or in vitro replicates:

3 females

Details on study design:

SCORING SYSTEM: Draize scoring system

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility not applicable

Irritation parameter:

iris score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

2

Reversibility:

other: reversibility not applicable

Irritation parameter:

conjunctivae score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

3

Reversibility:

other: reversibility not applicable

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

out of all three animals

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: reversibility not applicable

Irritant / corrosive response data:

No corneal or iridial effects were observed. Conjunctival effects included slight redness (1 animal) and slight discharge (1 animal) and were transiently seen approx. 1 h after dosing.

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

CLP: not classified
DSD: not classified

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin Irritation

CAS 68424-31-7

Three primary skin irritation studies were conducted to assess the skin irritation potential of Fatty acids, C5-10, esters with pentaerythritol (CAS 68424-31-7).

In the first primary skin irritation study, which was performed according to OECD Guideline 404 (Robinson, 1991a), the shaved skin of three female New Zealand White rabbits was exposed to 0.5 mL unchanged test substance for 4 hours under semiocclusive conditions. Skin reactions were assessed 1, 24, 48, 72 and 96 hours after removal of the test substance. The exposure period of 4 hours caused slight edema (grade = 1) in all animals 24 hours after removal of the test substance. The effect fully reversed within 48 hours. Erythema was seen at the test site of one animal (grade 2) and slightly at the test site of the other two animals (grade =1). Erythema reactions were completely reversed within 96 hours after removal of the test substance in all three animals. The mean erythema score over 24, 48 and 72 hours was 1.3 for animal #1 and #3 and 1.7 for animal #2. The mean edema score over 24, 48 and 72 hours was 0.3 for all three animals. All treated skin sites appeared normal at the 96 hour observation.

Two further studies, which were performed comparable to OECD Guideline 404, investigated primary dermal irritation of 0.5 mL test substance on the shaved skin of three male New Zealand White rabbits each for a single 4-hour application period (Robinson, 1991b + c). Skin reactions were assessed 1, 24, 48, 72, 96 hours and 7, 8 and 12 days after removal of the test substance. In the first study, moderate to severe erythema (maximum score = 4) and moderate to severe edema (maximum score = 4) were observed for 2 or 3 days after application in all animals. This regressed and no signs of erythema or edema were evident 4 to 12 days after application. Thickening of the skin at the application site was seen on one animal between 4 and 7 days after application. No other signs of irritations were observed. In the second study, very slight to well-defined erythema and very slight to slight oedema were observed in all animals The mean erythema score over 24, 48 and 72 hours was 1 for the first animal and 1.3 for the remaining two animals. The edema score over 24, 48 and 72 hours was 1 for the first animal and 1.7 for the remaining two animals. These reversed and no signs of erythema or oedema were evident 11 or 12 days after application. Slight desquamation at the application site of one animal was observed 7 days after application.

The test material is not considered irritating to the skin in with regard to the studies performed with Tetra-esterification products of C5, C7, C8, C10 fatty acids with pentraerythritol.

Eye Irritation

CAS 68424-31-7

Fatty acids, C5-10, esters with pentaerythritol (CAS 68424-31-7) was tested for its acute eye irritation potential in a study performed comparable to OECD Guideline 405 (Robinson, 1991d). The undiluted test material (0.1 mL) was applied into the conjunctival sac of three female New Zealand White rabbits each. The animals were observed for three days and reactions were assessed 1, 24, 48 and 72 hours after instillation according to the Draize scoring system. No corneal or iridial effects were observed. Conjunctival effects included slight redness (1 animal) and slight discharge (1 animal) and were transiently seen approximately 1 hour after dosing. Thus, the test material is not considered irritating to the eyes in this study.

Justification for classification or non-classification

All available data on skin and eye irritation/corrosion do not meet the classification criteria according to Regulation (EC) 1272/2008.