Tetrasodium 2,2''-diamino-8,8''-dihydroxy-4',4'''-{4-[bis(2-hydroxyethyl)amino]-1,3,5-triazine-2,6-diyldiimino}bis(naphthalene-1-azobenzene-2',6-disulfonate)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 12th to 23rd, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Red LF 6339

IUPAC Name:

Red LF 6339

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

samples were taken just before test start (duplicate samples from the single test medium and duplicate samples from the control)
after 48 and 96 hours - stability samples (duplicate samples from the single test medium and duplicate samples from the control)
all samples were taken from the approximate center of the aquaria without mixing of the test media.

Test solutions

Details on test solutions:

Stock solution of nominal 500 mg/l was prepared by dissolvig 1.501 g of the test item completely in 3 liters test water by sttirring for 10 min at RT.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Length at study initiation (length definition, mean, range and SD): 4.50 cm
- Weight at study initiation (mean and range, SD): 0.77 g
- Method of breeding: obtained from a trout breeding station Zeiningen, CH-4314, Switzerland

ACCLIMATION
- Acclimation period: more than 2 weeks
- Type and amount of food during acclimation: commercial fish diet
- Feeding frequency during acclimation: yes. until one day before the start of the test
- Health during acclimation (any mortality observed): mortality rate < 5 %. all fish were healthy

FEEDING DURING TEST: not fed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/l as CaCO3

Test temperature:

14 °C

pH:

7.7-7.9

Dissolved oxygen:

> 9.2 mg/l

Nominal and measured concentrations:

100 mg/l (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium with 1 liters test medium
- Aeration: slightly aerated during test. test water aerated prior to the preparation of the test medium until oxygen saturation was reached.
- No. of organisms per vessel: 7 fish
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- Biomass loading rate:< 1 g fish wet weight/litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4/1
- Intervals of water quality measurement: at the start of the test and once every day during the test.

OTHER TEST CONDITIONS
- Photoperiod: 16-hr light, 8-hr darkness. 30-minute transition period
- Light intensity: 50-500 lux

EFFECT PARAMETERS MEASURED: after 3, 24, 48, 72 and 96 hours for mortaliry and visible abnormalities. Due to an intense coloration of the test medium by the test item, a 500-W halogen lamp was used to determine the survival and visible abnormalities at the test fish during the test period in the test concentration of nominal 100 mg/l.

TEST CONCENTRATIONS
- Test concentrations: 100 mg/l

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

the test item is sufficiently stable in the test water under test conditions.
analytically determined mean test item concentration in the test medium varied from 93-108 % of the nominal value.
mean measured test item concentration corresponded to 102 % of the nominal value.
no visible abnormalities were observed at the test fish.
the single test solution was a clear solution colored dark-red .

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

LC50 (96 h) > 100 mg/l (nominal)