Tetrasodium 2,2''-diamino-8,8''-dihydroxy-4',4'''-{4-[bis(2-hydroxyethyl)amino]-1,3,5-triazine-2,6-diyldiimino}bis(naphthalene-1-azobenzene-2',6-disulfonate)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 12th to September 11th, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Red LF 6339

IUPAC Name:

Red LF 6339

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

samples were takedn just before test start (duplicate samples from the single test medium and duplicate samples from the control without daphnids)
after 48 hours - stability samples (duplicate samples from the single test medium and duplicate samples from the control

Test solutions

Details on test solutions:

test solution prepared by dissolving 30 mg test item completely in 300 ml test water by intense stirring for 10 minutes at RT

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain/clone: clone 5
- Source: originally supplied by the University of Sheffield/UK
- Age at the start of the test (mean and range, SD): 6-24 hrs old and are not first brood progeny
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/l as CaCO

Test temperature:

20-21 °C

pH:

7.9-8.0

Dissolved oxygen:

>8.0 mg/l

Nominal and measured concentrations:

100 mg/l (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 100-ml glass beaked filled with 50 ml test medium
- Type (delete if not applicable): covered with glass plates
- Aeration: test water aerated prior to the start of the study until oxygen saturation was reached. During the test period the test water was not aerated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): two
- No. of vessels per control (replicates): two
- Biomass loading rate: < one daphina per 2 ml test medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted test water
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4/1
- Culture medium different from test medium: no
- Intervals of water quality measurement: at the start and at the end of the test, pH values, the dissolved oxygen concentrations and the water temperature were determined in the single test concentration and the control. The appearance of the test medium was visually recorded at the start of the test and after 24 and 48 hours.

OTHER TEST CONDITIONS
- Photoperiod: 16-hr light and 8 hr darkness with 30 minute transition period
- Light intensity: 550-685 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : daphnids were observed form immobility after 24 and 48 hrs of exposure

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

the test item is suffuciently stable in the test water under test conditions.
analytically determined mean test item concentration in the test medium varied from 100-98 % of the nominal value.
mean measured test item concentration corresponded to 99 % of the nominal value
No remarkable observations were made concerning the appearance of the test medium
the test item has no toxic effect on Daphnia magna

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

NOEC(48h) = 100 mg/l
EC0(48h) = 100 mg/l
EC50(48h) >100 mg/l